Retro-orbital injections in mice.
TLDR
The authors report that they have routinely used retro-orbital injection of the venous sinus in the adult mouse to administer volumes up to 150 μl without incident and this technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds.Abstract:
Intravenous vascular access is technically challenging in the adult mouse and even more challenging in neonatal mice. The authors describe the technique of retro-orbital injection of the venous sinus in the adult and neonatal mouse. This technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds. The authors report that they have routinely used this technique in the adult mouse to administer volumes up to 150 μl without incident. Administration of retro-orbital injections is more challenging in neonatal mice but can reliably deliver volumes up to 10 μl.read more
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Pathological application of carbocyanine dye-based multicolour imaging of vasculature and associated structures
TL;DR: This work describes a histochemical strategy for three-dimensional, multicolour imaging of vasculature and associated structures, using a carbocyanine dye-based technique, vessel painting, and developed a series of applications to allow the combination of vessel painting with other histochemical methods, including immunostaining and tissue clearing for confocal and two-photon microscopies.
BookDOI
Quantum dots : applications in biology
Adriana Fontes,Beate S. Santos +1 more
TL;DR: Part I: Tutorials 1.
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In Vivo Characterization of Platinum(II)-Based Linker Technology for the Development of Antibody-Drug Conjugates: Taking Advantage of Dual Labeling with 195mPt and 89Zr.
Joey A. Muns,Veronica Montserrat,Hendrik Jan Houthoff,Karlijn Codee-van der Schilden,Oene Zwaagstra,Niels J. Sijbrandi,Eugen Merkul,Guus A.M.S. van Dongen +7 more
TL;DR: This study aimed to characterize Lx-based ADCs for in vivo stability and tumor targeting using 195mPt and 89Zr and determined that ADCs containing the Lx linker are stable in vivo.
Journal ArticleDOI
Lymphatic Specific Disruption in the Fine Structure of Heparan Sulfate Inhibits Dendritic Cell Traffic and Functional T Cell Responses in the Lymph Node
Xin Yin,Scott C. Johns,Scott C. Johns,Daniel Kim,Daniel Kim,Zbigniew Mikulski,Catherina L. Salanga,Tracy M. Handel,Monica Macal,Elina I. Zuniga,Mark M. Fuster,Mark M. Fuster +11 more
TL;DR: It is demonstrated that genetically altering the fine structure (N-sulfation) of heparan sulfate specifically in mouse lymphatic endothelium significantly reduces DC trafficking to regional LNs in vivo, and validates thefine structure of lymphatic vascular-specific HS as a novel molecular target for strategies aiming to modulate DC behavior and/or alter pathologic T cell responses in lymph nodes.
Journal ArticleDOI
Differential contribution of excitatory and inhibitory neurons in shaping neurovascular coupling in different epileptic neural states
TL;DR: The findings provide useful insights into how perfusion signals of different epileptic states are related in terms of NVC, and suggest that excitatory and inhibitory neurons differentially contribute to shaping the ictal and preictal neural states, respectively.
References
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The Mouse in biomedical research
James G. Fox,Stephen W. Barthold,Muriel T. Davisson,Christian E. Newcomer,Fred W. Quimby,Abigail L Smith +5 more
TL;DR: The Mouse in biomedical research , The Mouse inomedical research , مرکز فناوری اطلاعات و £1,000,000; اوشاوρزی; کسورز رسانی ;
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Mammalian Mst1 and Mst2 kinases play essential roles in organ size control and tumor suppression
Hai Song,Kinglun Kingston Mak,Lilia Topol,Kangsun Yun,Jianxin Hu,Lisa Garrett,Yongbin Chen,Ogyi Park,Jia Chang,R. Mark Simpson,Cun-Yu Wang,Bin Gao,Jin Jiang,Yingzi Yang +13 more
TL;DR: It is found that Mst1 and Mst2, the two mouse homologs of the Drosophila Hpo, control the sizes of some, but not all organs, in mice, and MSt1 andMst2 act as tumor suppressors by restricting cell proliferation and survival.
Journal ArticleDOI
Optimal Germinal Center Responses Require a Multistage T Cell:B Cell Adhesion Process Involving Integrins, SLAM-Associated Protein, and CD84
Jennifer L. Cannons,Hai Qi,Kristina T. Lu,Mala Dutta,Mala Dutta,Julio Gomez-Rodriguez,Jun Cheng,Edward K. Wakeland,Ronald N. Germain,Pamela L. Schwartzberg +9 more
TL;DR: The results reveal insight into the dynamic regulation of T cell:B cell interactions and identify SLAM family members as critical components of sustained T cell-B cell adhesion required for productive humoral immunity.
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The laboratory mouse
TL;DR: This chapter discusses the development of Phenotyping Terminology and Tests, and some of the techniques used to develop and evaluate these techniques.