Two separate gene clusters encode the biosynthetic pathway for the meroterpenoids, austinol and dehydroaustinol in Aspergillus nidulans
Hsien-Chun Lo,Ruth Entwistle,Chun-Jun Guo,Manmeet Ahuja,Edyta Szewczyk,Jui-Hsiang Hung,Yi-Ming Chiang,Berl R. Oakley,Clay C. C. Wang +8 more
TLDR
Chemical analysis of mutant extracts enabled us to isolate 3,5-dimethylorsellinic acid and 10 additional meroterpenoids that are either intermediates or shunt products from the biosynthetic pathway, and allow us to propose a complete biosynthesis pathway for the A. nidulans meroter penoids.Abstract:
Meroterpenoids are a class of fungal natural products that are produced from polyketide and terpenoid precursors. An understanding of meroterpenoid biosynthesis at the genetic level should facilitate engineering of second-generation molecules and increasing production of first-generation compounds. The filamentous fungus Aspergillus nidulans has previously been found to produce two meroterpenoids, austinol and dehydroaustinol. Using targeted deletions that we created, we have determined that, surprisingly, two separate gene clusters are required for meroterpenoid biosynthesis. One is a cluster of four genes including a polyketide synthase gene, ausA. The second is a cluster of 10 additional genes including a prenyltransferase gene, ausN, located on a separate chromosome. Chemical analysis of mutant extracts enabled us to isolate 3,5-dimethylorsellinic acid and 10 additional meroterpenoids that are either intermediates or shunt products from the biosynthetic pathway. Six of them were identified as novel me...read more
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Regulation of fungal secondary metabolism
TL;DR: This Review describes the current understanding of the regulatory elements that modulate the transcription of genes involved in secondary metabolism and discusses how an improved knowledge of these regulatory elements will ultimately lead to a betterUnderstanding of the physiological and ecological functions of these important compounds.
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Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus
Ronald P. de Vries,Robert Riley,Ad Wiebenga,Guillermo Aguilar-Osorio,Sotiris Amillis,Cristiane Uchima,Gregor Anderluh,Mojtaba Asadollahi,Marion Askin,Marion Askin,Kerrie Barry,Evy Battaglia,Özgür Bayram,Özgür Bayram,Tiziano Benocci,Susanna A. Braus-Stromeyer,Camila Caldana,David Cánovas,David Cánovas,Gustavo C. Cerqueira,Fusheng Chen,Wanping Chen,Cindy Choi,Alicia Clum,Renato Augusto Corrêa dos Santos,André Damasio,George Diallinas,Tamás Emri,Erzsébet Fekete,Michel Flipphi,Susanne Freyberg,Antonia Gallo,Christos Gournas,Rob Habgood,Matthieu Hainaut,María Harispe,Bernard Henrissat,Bernard Henrissat,Bernard Henrissat,Kristiina Hildén,Ryan Hope,Abeer Hossain,Eugenia Karabika,Eugenia Karabika,Levente Karaffa,Zsolt Karányi,Nada Kraševec,Alan Kuo,Harald Kusch,Kurt LaButti,Ellen Lagendijk,Alla Lapidus,Alla Lapidus,Anthony Levasseur,Erika Lindquist,Anna Lipzen,Antonio F. Logrieco,Andrew MacCabe,Miia R. Mäkelä,Iran Malavazi,Petter Melin,Vera Meyer,Natalia Mielnichuk,Natalia Mielnichuk,Márton Miskei,Ákos Molnár,Giuseppina Mulè,Chew Yee Ngan,Margarita Orejas,Erzsébet Orosz,Erzsébet Orosz,Jean Paul Ouedraogo,Jean Paul Ouedraogo,Karin M. Overkamp,Hee-Soo Park,Giancarlo Perrone,François Piumi,François Piumi,Peter J. Punt,Arthur F. J. Ram,Ana Ramón,Stefan Rauscher,Eric Record,Diego Mauricio Riaño-Pachón,Vincent Robert,Julian Röhrig,Roberto Ruller,Asaf Salamov,Nadhira Salih,Nadhira Salih,Rob Samson,Erzsébet Sándor,Manuel Sanguinetti,Tabea Schütze,Tabea Schütze,Kristina Sepčić,Ekaterina Shelest,Gavin Sherlock,Vicky Sophianopoulou,Fabio M. Squina,Hui Sun,Antonia Susca,Richard B. Todd,Adrian Tsang,Shiela E. Unkles,Nathalie van de Wiele,Diana van Rossen-Uffink,Juliana Velasco de Castro Oliveira,Tammi Camilla Vesth,Jaap Visser,Jae-Hyuk Yu,Miaomiao Zhou,Mikael Rørdam Andersen,David B. Archer,Scott E. Baker,Isabelle Benoit,Isabelle Benoit,Axel A. Brakhage,Gerhard H. Braus,Reinhard Fischer,Jens Christian Frisvad,Gustavo H. Goldman,Jos Houbraken,Berl R. Oakley,István Pócsi,Claudio Scazzocchio,Claudio Scazzocchio,Bernhard Seiboth,Patricia A. vanKuyk,Patricia A. vanKuyk,Jennifer R. Wortman,Paul S. Dyer,Igor V. Grigoriev +132 more
TL;DR: In this article, a comparative genomics and experimental study of the aspergilli genus is presented, which allows for the first time a genus-wide view of the biological diversity of the Aspergillus and in many, but not all, cases linked genome differences to phenotype.
Journal ArticleDOI
Biosynthesis of fungal meroterpenoids
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Oxidative Cyclization in Natural Product Biosynthesis
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References
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Tania Nayak,Edyta Szewczyk,C. Elizabeth Oakley,Aysha H. Osmani,Leena Ukil,Sandra L. Murray,Michael J. Hynes,Stephen A. Osmani,Berl R. Oakley +8 more
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Journal ArticleDOI
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Journal ArticleDOI
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Daren W. Brown,Jae-Hyuk Yu,Jae-Hyuk Yu,Hemant S. Kelkar,Mary Fernandes,T C Nesbitt,Nancy P. Keller,Thomas H. Adams,Thomas J. Leonard,Thomas J. Leonard +9 more
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Journal ArticleDOI
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