Pierre-and-Marie-Curie University

About: Pierre-and-Marie-Curie University is a education organization based out in Paris, France. It is known for research contribution in the topics: Population & Raman spectroscopy. The organization has 34448 authors who have published 56139 publications receiving 2392398 citations.

Does the high nucleic acid content of individual bacterial cells allow us to discriminate between active cells and inactive cells in aquatic systems

Abstract: The nucleic acid contents of individual bacterial cells as determined with three different nucleic acid-specific fluorescent dyes (SYBR I, SYBR II, and SYTO 13) and flow cytometry were compared for different seawater samples. Similar fluorescence patterns were observed, and bacteria with high apparent nucleic acid contents (HNA) could be discriminated from bacteria with low nucleic acid contents (LNA). The best discrimination between HNA and LNA cells was found when cells were stained with SYBR II. Bacteria in different water samples collected from seven freshwater, brackish water, and seawater ecosystems were prelabeled with tritiated leucine and then stained with SYBR II. After labeling and staining, HNA, LNA, and total cells were sorted by flow cytometry, and the specific activity of each cellular category was determined from leucine incorporation rates. The HNA cells were responsible for most of the total bacterial production, and the specific activities of cells in the HNA population varied between samples by a factor of seven. We suggest that nucleic acid content alone can be a better indicator of the fraction of growing cells than total counts and that this approach should be combined with other fluorescent physiological probes to improve detection of the most active cells in aquatic systems.

QCD Predictions for Charm and Bottom Quark Production at RHIC

Abstract: We make up-to-date QCD predictions for open charm and bottom production at RHIC in nucleon-nucleon collisions at {radical}S = 200 GeV. We also calculate the electron spectrum resulting from heavy flavor decays to allow direct comparison to the data. A rigorous benchmark, including the theoretical uncertainties, is established against which nuclear collision data can be compared to obtain evidence for nuclear effects.

Thin crust, ultramafic exposures, and rugged faulting patterns at the Mid-Atlantic Ridge (22°–24°N)

Abstract: Off-axis rock sampling in the lat 22°–24° N region of the Mid-Atlantic Ridge shows that the emplacement of mantle-derived rocks in the sea floor has been a common process there for the past few million years. We find a good correlation between domains of positive residual gravity anomalies (inferred to have a thin crust) and the distribution of ultramafic samples. We also find that thin-crust domains have a rugged topography, thought to reflect strong tectonic disruption. We propose that these thin-crust domains are made of tectonically uplifted ultramafic rocks, with gabbroic intrusions and a thin basaltic cover. We also suggest that strong tectonic disruption may be a direct consequence of the lithological and rheological heterogeneity of these thin-crust domains.

Quantum Black Holes, Wall Crossing, and Mock Modular Forms

Abstract: We show that the meromorphic Jacobi form that counts the quarter-BPS states in N=4 string theories can be canonically decomposed as a sum of a mock Jacobi form and an Appell-Lerch sum. The quantum degeneracies of single-centered black holes are Fourier coefficients of this mock Jacobi form, while the Appell-Lerch sum captures the degeneracies of multi-centered black holes which decay upon wall-crossing. The completion of the mock Jacobi form restores the modular symmetries expected from $AdS_3/CFT_2$ holography but has a holomorphic anomaly reflecting the non-compactness of the microscopic CFT. For every positive integral value m of the magnetic charge invariant of the black hole, our analysis leads to a special mock Jacobi form of weight two and index m, which we characterize uniquely up to a Jacobi cusp form. This family of special forms and another closely related family of weight-one forms contain almost all the known mock modular forms including the mock theta functions of Ramanujan, the generating function of Hurwitz-Kronecker class numbers, the mock modular forms appearing in the Mathieu and Umbral moonshine, as well as an infinite number of new examples.

Systems medicine: the future of medical genomics and healthcare

Abstract: High-throughput technologies for DNA sequencing and for analyses of transcriptomes, proteomes and metabolomes have provided the foundations for deciphering the structure, variation and function of the human genome and relating them to health and disease states. The increased efficiency of DNA sequencing opens up the possibility of analyzing a large number of individual genomes and transcriptomes, and complete reference proteomes and metabolomes are within reach using powerful analytical techniques based on chromatography, mass spectrometry and nuclear magnetic resonance. Computational and mathematical tools have enabled the development of systems approaches for deciphering the functional and regulatory networks underlying the behavior of complex biological systems. Further conceptual and methodological developments of these tools are needed for the integration of various data types across the multiple levels of organization and time frames that are characteristic of human development, physiology and disease. Medical genomics has attempted to overcome the initial limitations of genome-wide association studies and has identified a limited number of susceptibility loci for many complex and common diseases. Iterative systems approaches are starting to provide deeper insights into the mechanisms of human diseases, and to facilitate the development of better diagnostic and prognostic biomarkers for cancer and many other diseases. Systems approaches will transform the way drugs are developed through academy-industry partnerships that will target multiple components of networks and pathways perturbed in diseases. They will enable medicine to become predictive, personalized, preventive and participatory, and, in the process, concepts and methods from Western and oriental cultures can be combined. We recommend that systems medicine should be developed through an international network of systems biology and medicine centers dedicated to inter-disciplinary training and education, to help reduce the gap in healthcare between developed and developing countries.