Wuhan University

About: Wuhan University is a education organization based out in Wuhan, China. It is known for research contribution in the topics: Computer science & Population. The organization has 92849 authors who have published 92882 publications receiving 1691049 citations. The organization is also known as: WHU & Wuhan College.

Quantification of effects of climate variations and human activities on runoff by a monthly water balance model: A case study of the Chaobai River basin in northern China

Abstract: The Chaobai River basin in northern China consists of two major tributaries, the Chao River and Bai River. Monthly observations of precipitation, streamfiow, and panevaporation data are available for 35 years (1961-1966 and 1973-2001). Using the annual time series of the observed streamfiow, one break point at 1979 is detected and is adopted to divide the data set into two study periods, the "before" and "after" periods marking the onset of significant anthropogenic alteration of the flow (reservoirs and silt retention dams, five times increase in population) and significant changes in land use (conversion to terraced fields versus sloping fields). The distributed time-variant gain model (DTVGM) was used to evaluate the water resources of the area. Furthermore, the Bayesian method used by Engeland et al. (2005) was used in this paper to evaluate two uncertainty sources (i.e., the model parameter and model structure) and for assessing the DTVGM's performance over the Chaobai River basin. Comparing the annual precipitation means over 13 years (1961-1966 and 1973-1979), the means of the second period (1980-2001) decreased by 5.4% and 4.9% in the Chao River and Bai River basins, respectively. However, the related annual runoff decreased by 40.3% and 52.8%, respectively, a much greater decline than exhibited by precipitation. Through the monthly model simulation and the fixing-changing method, it is determined that decreases in runoff between the two periods can be attributed to 35% (31%) from climate variations and 68% (70%) from human activities in the Chao River (Bai River). Thus, human impact exerts a dominant influence upon runoff decline in the Chaobai River basin compared to climate. This study enhances our understanding of the relative roles of climate variations and human activities on runoff. © 2009 by the American Geophysical Union.

Genome-wide analysis identifies NR4A1 as a key mediator of T cell dysfunction.

Abstract: T cells become dysfunctional when they encounter self antigens or are exposed to chronic infection or to the tumour microenvironment1. The function of T cells is tightly regulated by a combinational co-stimulatory signal, and dominance of negative co-stimulation results in T cell dysfunction2. However, the molecular mechanisms that underlie this dysfunction remain unclear. Here, using an in vitro T cell tolerance induction system in mice, we characterize genome-wide epigenetic and gene expression features in tolerant T cells, and show that they are distinct from effector and regulatory T cells. Notably, the transcription factor NR4A1 is stably expressed at high levels in tolerant T cells. Overexpression of NR4A1 inhibits effector T cell differentiation, whereas deletion of NR4A1 overcomes T cell tolerance and exaggerates effector function, as well as enhancing immunity against tumour and chronic virus. Mechanistically, NR4A1 is preferentially recruited to binding sites of the transcription factor AP-1, where it represses effector-gene expression by inhibiting AP-1 function. NR4A1 binding also promotes acetylation of histone 3 at lysine 27 (H3K27ac), leading to activation of tolerance-related genes. This study thus identifies NR4A1 as a key general regulator in the induction of T cell dysfunction, and a potential target for tumour immunotherapy.

Psychological health, sleep quality, and coping styles to stress facing the COVID-19 in Wuhan, China.

Abstract: To understand Wuhan residents’ psychological reactions to the COVID-19 epidemic and offer a reference point for interventions, an online questionnaire survey was conducted. It included the Disorder 7-Item Scale (GAD-7), the Patient Health Questionnaire 9-Item Scale (PHQ-9), Athens Insomnia Scale, and Simplified Coping Style Questionnaire. Categorical data were reported as numbers and percentages. Multivariate logistic regression models were used to evaluate the association between demographic factors and anxiety, depression, sleep disorder, and passive coping style. A total of 1242 Wuhan residents investigated, 27.5% had anxiety, 29.3% had depression, 30.0% had a sleep disorder, and 29.8% had a passive response to COVID-19. Being female was the risk factor for anxiety (OR = 1.62) and sleep disorder (OR = 1.36); being married was associated with anxiety (OR = 1.75); having a monthly income between 1000 and 5000 CNY (OR = 1.44, OR = 1.83, OR = 2.61) or >5000 CNY (OR = 1.47, OR = 1.45, OR = 2.14) was a risk factor for anxiety, depression, and sleep disorder; not exercising (OR = 1.45, OR = 1.71, OR = 1. 85, OR = 1.71) was a common risk factor for anxiety, depression, sleep disorder, and passive coping style; and having a higher education level (bachelor’s degree and above) (OR = 1.40) was associated with having a sleep disorder. Wuhan residents’ psychological status and sleep quality were relatively poorer than they were before the COVID-19 epidemic; however, the rate of passive coping to stress was relatively higher.

Highly effective colorimetric and visual detection of nucleic acids using an asymmetrically split peroxidase DNAzyme.

Abstract: G-quadruplex containing peroxidase DNAzyme is a complex of hemin and a single-stranded guanine-rich DNA (hemin-binding DNA aptamer), which is used as an attractive catalytic label for biosensing recently. Therein, the hemin-binding DNA aptamer contains four GGG repeats and can fold into a G-quadruplex structure. In this paper, we have developed a new split mode to divide the hemin-binding DNA aptamer into two parts: one possesses three GGG repeats, and another part possesses one GGG repeat, namely, the 3:1 split mode. The combination of G-quadruplex and hemin binding could be used as a sensitive probe for the identification of single nucleotide polymorphisms by giving a color signal, visible to the naked eye at room temperature. The G-quadruplex containing peroxidase DNAzyme utilizes the 3:1 split mode and can be directly used for the identification of SNPs with a detection limit in the nM range when the matching length of the probe is short enough. When the matching length of the probe is relatively long...

Osteoinduction A Review of Old Concepts with New Standards

Abstract: Since the discovery of osteoinduction in the early 20th century, innovative biomaterials with osteoinductive potential have emerged. Over the last 50 years, however, our ability to describe biological phenomena accurately has been improved dramatically by advancements in cell and molecular biology. The aim of this review is to divide the osteoinduction phenomenon into 3 principles: (1) mesenchymal cell recruitment, (2) mesenchymal differentiation to bone-forming osteoblasts, and (3) ectopic bone formation in vivo. Furthermore, this review formulates guidelines for in vitro and in vivo experimental testing for accurately defining new biomaterials as osteoinductive. The use of growth factors with osteoinductive potential in periodontal and oral surgery is discussed. These concepts and guidelines aim to guide the future direction of emerging biomaterials in bone regeneration.