A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
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Gut health: The results of microbial and mucosal immune interactions in pigs.
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TL;DR: This review discusses the interaction mechanism between the flora and intestinal mucosal immunity, as well as the regulation through fecal microbiota transplantation (FMT), dietary nutritional composition, probiotics and prebiotics of pig intestinal microecology and provides insights into the relationship between intestinal microorganisms and the mucosal immune system.
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Human colon function ex vivo: Dependence on oxygen and sensitivity to antibiotic.
TL;DR: A human colon organotypic slice model was established for applications ranging from gut epithelial proliferation to enteric pathogen influence on mucosal immune functions ex vivo, which further support the need to account for oxygen concentration in primary tissue cultures, and that antibiotic use impacts gut-microbe-immune interactions.
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What can microfluidics do for human microbiome research
Hsih Yin Tan,Yi-Chin Toh +1 more
TL;DR: The goal of this paper is to discuss and highlight the opportunities and unique challenges that microfluidics must overcome when working with microbiome-relevant biological materials, e.g., micro-organisms, host tissues, and fluids.
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Contributions of the microbiome to intestinal inflammation in a gut-on-a-chip
Min Seo Jeon,Yoon Young Choi,Sung Jun Mo,Jang Ho Ha,Young Seo Lee,Hee Uk Lee,Soo-Dong Park,Jae Jung Shim,Jung Lyoul Lee,Bong Geun Chung +9 more
TL;DR: In this article , a microfluidic-based Gut-On-a-Chip (GOC) model is presented, which allows co-culture of human and microbial cells to mimic the gastrointestinal structure.
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Bioengineering approaches to simulate human colon microbiome ecosystem
TL;DR: There is a significant opportunity for further improvement of the models’ experimental setups towards more realistic operating systems, including mucosal surfaces, intestinal cells and tissues allowing microbiome–host crosstalk representation.
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