A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
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In Vitro Models of the Small Intestine: Engineering Challenges and Engineering Solutions
Sarah A Hewes,Reid L. Wilson,Reid L. Wilson,Mary K. Estes,Noah F. Shroyer,Sarah E. Blutt,K. Jane Grande-Allen +6 more
TL;DR: An overview of the complexity of small intestinal anatomy and bioengineered models that recapitulate some of these physiological aspects is presented.
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Organ-on-a-Chip Systems for Women's Health Applications.
TL;DR: An introduction into preclinical and clinical research on women's health is given and where organ‐on‐a‐chip systems are already utilized or have the potential to deliver new insights and enable entirely new types of studies are discussed.
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Artificial Microbial Arenas: Materials for Observing and Manipulating Microbial Consortia
Lothar Wondraczek,Georg Pohnert,Georg Pohnert,Felix H. Schacher,Angela Köhler,Angela Köhler,Michael Gottschaldt,Ulrich S. Schubert,Kirsten Küsel,Kirsten Küsel,Kirsten Küsel,Axel A. Brakhage,Axel A. Brakhage +12 more
TL;DR: A perspective on how materials research will play an essential role in generating answers to the most pertinent questions of microbial engineering is presented, and the concept of adaptive microbial arenas and possibilities for their construction from particulate microniches to 3D habitats is introduced.
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Organoid-based Models to Study the Role of Host-microbiota Interactions in IBD.
TL;DR: The role of host-microbiota interactions in disease onset and progression is pivotal, and requires representative models mimicking the gastrointestinal ecosystem, including the intestinal epithelium, the gut microbiota, and immune cells.
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Models of the Gut for Analyzing the Impact of Food and Drugs
Chiara Anna Maria Fois,Thi Yen Loan Le,Aaron Schindeler,Sina Naficy,Dale D. McClure,Mark Read,Peter Valtchev,Ali Khademhosseini,Fariba Dehghani +8 more
TL;DR: It is posited that only through complementary use of these models will salient research questions be able to be addressed, and how in silico approaches may have utility in predicting and interpreting experimental data is discussed.
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