A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
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SARS-CoV-2 induced intestinal responses with a biomimetic human gut-on-chip.
Yaqiong Guo,Yaqiong Guo,Rong-Hua Luo,Yaqing Wang,Yaqing Wang,Pengwei Deng,Pengwei Deng,Tian-Zhang Song,Min Zhang,Min Zhang,Peng Wang,Xu Zhang,Kangli Cui,Kangli Cui,Tingting Tao,Tingting Tao,Zhongyu Li,Wenwen Chen,Wenwen Chen,Yong-Tang Zheng,Yong-Tang Zheng,Jianhua Qin +21 more
TL;DR: An intestinal infection model on a chip is created that allows the recapitulation of human relevant intestinal pathophysiology induced by SARS-CoV-2 at organ level and provides a unique and rapid platform to accelerate COVID-19 research and develop novel therapies.
Journal ArticleDOI
From Network Analysis to Functional Metabolic Modeling of the Human Gut Microbiota
Eugen Bauer,Ines Thiele +1 more
TL;DR: This minireview will discuss current approaches in systems biology to analyze the human gut microbiota, with a particular focus on constraint-based modeling.
Journal ArticleDOI
Towards the development of human immune-system-on-a-chip platforms.
Alessandro Polini,Loretta L. del Mercato,Adriano Barra,Yu Shrike Zhang,F. Calabi,Giuseppe Gigli +5 more
TL;DR: Some distinctive features of the immune system are recapitulate before reviewing microfluidic devices that mimic lymphoid organs or other organs and/or tissues with an integrated immune system component.
Journal ArticleDOI
Organic transistor platform with integrated microfluidics for in-line multi-parametric in vitro cell monitoring.
Vincenzo F. Curto,Bastien Marchiori,Adel Hama,Anna-Maria Pappa,Magali Ferro,Marcel Braendlein,Jonathan Rivnay,Jonathan Rivnay,Michel Fiocchi,George G. Malliaras,Marc Ramuz,Róisín M. Owens +11 more
TL;DR: This platform will enable high-content screening for in vitro drug discovery and toxicology testing and bridges the existing gap in the integration of in-line sensors in microfluidic devices.
Journal ArticleDOI
Integrated culturing, modeling and transcriptomics uncovers complex interactions and emergent behavior in a three-species synthetic gut community.
Kevin D'hoe,Stefan Vet,Stefan Vet,Karoline Faust,Frédéric Moens,Gwen Falony,Didier Gonze,Verónica Lloréns-Rico,Lendert Gelens,Jan Danckaert,Luc De Vuyst,Jeroen Raes,Jeroen Raes +12 more
TL;DR: This work established a synthetic community composed of three representative human gut isolates and explored their interactions under well-controlled conditions in vitro, demonstrating that strains grown in co-culture behaved differently than those in mono-culture and confirming their altered behavior at the transcriptional level.
References
More filters
Journal ArticleDOI
limma powers differential expression analyses for RNA-sequencing and microarray studies
Matthew E. Ritchie,Belinda Phipson,Di Wu,Yifang Hu,Charity W. Law,Wei Shi,Gordon K. Smyth,Gordon K. Smyth +7 more
TL;DR: The philosophy and design of the limma package is reviewed, summarizing both new and historical features, with an emphasis on recent enhancements and features that have not been previously described.
Journal ArticleDOI
Linear Models and Empirical Bayes Methods for Assessing Differential Expression in Microarray Experiments
TL;DR: The hierarchical model of Lonnstedt and Speed (2002) is developed into a practical approach for general microarray experiments with arbitrary numbers of treatments and RNA samples and the moderated t-statistic is shown to follow a t-distribution with augmented degrees of freedom.
Journal ArticleDOI
R: A Language for Data Analysis and Graphics
Ross Ihaka,Robert Gentleman +1 more
TL;DR: In this article, the authors discuss their experience designing and implementing a statistical computing language, which combines what they felt were useful features from two existing computer languages, and they feel that the new language provides advantages in the areas of portability, computational efficiency, memory management, and scope.
Journal ArticleDOI
Diet rapidly and reproducibly alters the human gut microbiome
Lawrence A. David,Corinne F. Maurice,Rachel N. Carmody,David B. Gootenberg,Julie E. Button,Benjamin E. Wolfe,Alisha V. Ling,A. Sloan Devlin,Yug Varma,Michael A. Fischbach,Sudha B. Biddinger,Rachel J. Dutton,Peter J. Turnbaugh +12 more
TL;DR: Increases in the abundance and activity of Bilophila wadsworthia on the animal-based diet support a link between dietary fat, bile acids and the outgrowth of microorganisms capable of triggering inflammatory bowel disease.
Journal Article
HIV-1 Entry Cofactor: Functional cDNA Cloning of a Seven-Transmembrane, G Protein–Coupled Receptor
TL;DR: Fusin this article is a putative G protein-coupled receptor with seven transmembrane segments, which enabled CD4-expressing nonhuman cell types to support HIV-1 Env-mediated cell fusion and infection.