A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
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Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction.
Bolin Jing,Zhuo Wang,Chen Zhang,Deng Quanfeng,J. Y. Wei,Yong Luo,Zhang Xiuli,Jian-Jun Li,Yuguang Du +8 more
TL;DR: This human gut-vessel microfluidic system showed a good potential for investigating the host–microbial interaction and the effect and mechanism of microbiome on intestinal diseases in vitro.
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Gut microbiome a promising target for management of respiratory diseases
Riddhi Trivedi,Kalyani Barve +1 more
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Which experimental systems should we use for human microbiome science
TL;DR: This essay argues for thoughtful choice of model systems for human microbiome science, arguing for a greater variety of experimental systems, including wider use of invertebrate models, to benefit biomedical research.
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3D-Printed electrochemical sensor-integrated transwell systems.
Pradeep Ramiah Rajasekaran,Ashley A. Chapin,David N. Quan,Jens Herberholz,William E. Bentley,Reza Ghodssi +5 more
TL;DR: The team, led by Reza Ghodssi of the University of Maryland, added electrochemical sensors to the porous membrane normally found in transwell systems, enabling them to measure characteristics of the cultures, and developed a novel system which can measure cellular and molecular events in cell cultures non-invasively and in real-time.
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Application of Microfluidics in Experimental Ecology: The Importance of Being Spatial.
TL;DR: This mini review aims to demonstrate the versatility of microfluidics and the diversity of its applications that help the advance of microbiology, and in more general, experimental ecology.
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