A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
Citations
More filters
Journal ArticleDOI
The Human Gut Microbiome: From Association to Modulation
TL;DR: The type of studies that will be essential for translating microbiome research into targeted modulations with dedicated benefits for the human host are discussed.
Journal ArticleDOI
Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids
Magdalena Kasendra,Alessio Tovaglieri,Alessio Tovaglieri,Alexandra Sontheimer-Phelps,Alexandra Sontheimer-Phelps,Sasan Jalili-Firoozinezhad,Sasan Jalili-Firoozinezhad,Amir Bein,Angeliki Chalkiadaki,William Scholl,Cheng Zhang,Hannah Rickner,Camilla A. Richmond,Hu Li,David T. Breault,David T. Breault,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +18 more
TL;DR: The Intestine Chip may be useful as a research tool for applications where normal intestinal function is crucial, including studies of metabolism, nutrition, infection, and drug pharmacokinetics, as well as personalized medicine.
Journal ArticleDOI
A Complex Human-Gut Microbiome Cultured in an Anaerobic Intestine-on-a-Chip
Sasan Jalili-Firoozinezhad,Sasan Jalili-Firoozinezhad,Francesca S. Gazzaniga,Francesca S. Gazzaniga,Elizabeth Calamari,Diogo M. Camacho,Cicely W. Fadel,Amir Bein,Ben Swenor,Bret Nestor,Michael J. Cronce,Alessio Tovaglieri,Alessio Tovaglieri,Oren Levy,Katherine E. Gregory,Katherine E. Gregory,David T. Breault,David T. Breault,Joaquim M. S. Cabral,Dennis L. Kasper,Richard M. Novak,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +23 more
TL;DR: The extended coculture of living human intestinal epithelium with stable communities of aerobic and anaerobic human gut microbiota is shown, using a microfluidic intestine-on-a-chip that permits the control and real-time assessment of physiologically relevant oxygen gradients.
Journal ArticleDOI
Organs on a chip: a fast-track for engineered human tissues in drug development
TL;DR: The design considerations for single and multi-organ Oocs are reviewed, remaining challenges are discussed, and the potential impact of OOCs as a fast-track opportunity for tissue engineering to advance drug development and precision medicine is highlighted.
Journal ArticleDOI
Microfluidic Organ-on-a-Chip Models of Human Intestine.
Amir Bein,Woojung Shin,Sasan Jalili-Firoozinezhad,Sasan Jalili-Firoozinezhad,Min Hee Park,Alexandra Sontheimer-Phelps,Alexandra Sontheimer-Phelps,Alessio Tovaglieri,Alessio Tovaglieri,Angeliki Chalkiadaki,Hyun-Jung Kim,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +13 more
TL;DR: How microfluidic Intestine Chips offer new capabilities not possible with conventional culture systems or organoid cultures, including the ability to analyze contributions of individual cellular, chemical, and physical control parameters one-at-a-time is described.
References
More filters
Journal ArticleDOI
Enhanced tumorigenic potential of colorectal cancer cells by extracellular sulfatases.
Carolina M. Vicente,Marcelo A. Lima,Marcelo A. Lima,Edwin A. Yates,Edwin A. Yates,Helena B. Nader,Leny Toma +6 more
TL;DR: It is revealed that sulfatases have oncogenic effects in colon cancer cells, suggesting an important role for these enzymes in cancer progression.
Journal ArticleDOI
PILRα and PILRβ have a siglec fold and provide the basis of binding to sialic acid
Qiong Lu,Guangwen Lu,Jianxun Qi,Han Wang,Yifang Xuan,Qihui Wang,Yan Li,Yanfang Zhang,Chunfu Zheng,Zheng Fan,Jinghua Yan,George F. Gao +11 more
TL;DR: The molecular basis on the ligand binding of PILRs by systematic structural and functional assays was elucidated and key residues responsible for SA binding were identified and the atomic interaction details via a complex crystal structure were elucidated.
Journal ArticleDOI
Substance P is essential for maintaining gut muscle contractility: a novel role for coneurotransmission revealed by botulinum toxin.
TL;DR: A novel role for SP is identified as a coneurotransmitter that appears to be important for the maintenance of muscular responsiveness to the principal excitatory neurotransmitter, ACh.
Journal ArticleDOI
The NHE1 Na+/H+ exchanger regulates cell survival by activating and targeting ezrin to specific plasma membrane domains.
TL;DR: It is concluded that cell stresses promote NHE1-ezrin interaction, which activate cell survival pathways to prevent apoptosis in diabetic and non-diabetic kidney diseases.
Journal ArticleDOI
Autoinhibition of endothelial nitric oxide synthase (eNOS) in gut smooth muscle by nitric oxide.
John R. Grider,Karnam S. Murthy +1 more
TL;DR: NO produced in smooth muscle cells as a result of the activation of eNOS by VIP exerts an autoinhibitory restraint on eNos thereby regulating the balance of the VIP/cAMP/PKA and NO/cGMP/PKG pathways that regulate the relaxation of gut smooth muscle.