A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
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Emulating the gut-liver axis: Dissecting the microbiome's effect on drug metabolism using multiorgan-on-chip models.
TL;DR: In vitro multiorgan platforms, tailored for an individual patient's microbial background, could be used as a tool to predict the effect of the gut microbiome on pharmacokinetics in a personalized way and overcome current challenges in drug toxicity assessment assays.
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Dynamic metabolic interactions and trophic roles of human gut microbes identified using a minimal microbiome exhibiting ecological properties
Sudarshan A. Shetty,Ioannis Kostopoulos,Sharon Y. Geerlings,Hauke Smidt,Willem M. de Vos,Clara Belzer +5 more
TL;DR: In this paper , the authors designed a synthetic minimal microbial community (MDb-MM) by combining 16S rRNA gene-based composition analysis, metabolite measurements and metatranscriptomics to investigate community dynamics, stability, inter-species metabolic interactions and their trophic roles.
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Emerging Roles of Microfluidics in Brain Research: From Cerebral Fluids Manipulation to Brain-on-a-Chip and Neuroelectronic Devices Engineering.
TL;DR: This review starts with a critical discussion of the current understanding of several key topics in brain research such as neurovascular coupling, glymphatic pathway, and GBA and interrogates a wide range of microfluidic-based approaches that have been developed or can be improved to advance the fundamental understanding of brain functions.
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Simulation and modeling of dietary changes in the infant gut microbiome.
TL;DR: The impact of dietary changes in the gut microbiome is highlighted, and a modeling framework to predict this influence is provided, and the outcome after 48 h after each regime is represented.
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An in vitro model maintaining taxon-specific functional activities of the gut microbiome
Leyuan Li,Elias Abou-Samra,Zhibin Ning,Xu Zhang,Janice Mayne,Janet Wang,Kai Cheng,Krystal Walker,Alain Stintzi,Daniel Figeys,Daniel Figeys +10 more
TL;DR: The Mipro model provides a highly simulated gut microbiome for high-throughput investigation of drug-microbiome interactions and exhibited a high degree of in vitro – in vivo correlation in microbial responses to metformin in mice fed a high-fat diet.
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