A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
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Gut-on-a-Chip Research for Drug Development: Implications of Chip Design on Preclinical Oral Bioavailability or Intestinal Disease Studies
TL;DR: GOCs have been widely used in preclinical drug development research as mentioned in this paper , and many different GOC models have been presented over the past few years, and they offer more translational value compared to conventional in vitro models.
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Biosensors for point-of-care testing and personalized monitoring of gastrointestinal microbiota
TL;DR: In this article , the authors have discussed the significance and recent advancements of biosensors in monitoring the GI microbiota and highlighted the progress on future biosensing techniques such as lab-on-chip, smart materials, ingestible capsules, wearable devices, and fusion of machine learning/artificial intelligence (ML/AI) have also been highlighted.
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A 3D Bioprinted Gut Anaerobic Model for Studying Bacteria–Host Interactions
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TL;DR: In this article , a 3D model of the human intestinal epithelium has been developed to recapitulate the low oxygen concentrations in the intestinal lumen, which has been highlighted in recent years.
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Dissecting Human Gut Microbiome to Better Decipher Drug liability: a once-forgotten organ takes center stage.
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Anaerobic microbiota facilitate P. aeruginosa access to the airway epithelium in a novel co-culture model of colonization
Patrick J. Moore,Talia D. Wiggen,Leslie A. Kent,S. Arif,Sarah K. Lucas,O’Grady Sm,Ryan C. Hunter +6 more
TL;DR: In this paper, a dual oxic-anoxic co-culture approach was used to maintain an oxygen-limited apical epithelial microenvironment while host cells were oxygenated basolaterally.
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