scispace - formally typeset
Open AccessJournal ArticleDOI

A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.

TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.
Abstract
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)

read more

Content maybe subject to copyright    Report

Citations
More filters
Journal ArticleDOI

3D Immunocompetent Organ-on-a-Chip Models.

TL;DR: The different components of the immune system are discussed, as well as immune OOC platforms and immunocompetent OOC approaches that have simulated one or more components ofThe immune system.
Journal ArticleDOI

Beyond Tissue Stiffness and Bioadhesivity: Advanced Biomaterials to Model Tumor Microenvironments and Drug Resistance

TL;DR: It is discussed that new preclinical ex vivo models should consider a more comprehensive milieu of tissue signaling, nuclear mechanics, immune response, and the gut microbiome, to better understand tumorigenesis and to design therapeutic approaches to overcome resistance.
Journal ArticleDOI

Biomedical Application of Functional Materials in Organ-on-a-Chip.

TL;DR: This mini review provides a summary of the various type of materials for OOC systems, focusing on the progress of materials and related fabrication technologies within the last 5 years.
Journal ArticleDOI

Evaluating Human Intestinal Cell Lines for Studying Dietary Protein Absorption

TL;DR: An overview of the presence and activities of peptide- and amino acid transporters in these cell models and their suitability in screening novel food proteins and prediction of the nutritional quality needed for inclusion in the human diet of the future is addressed.
Journal ArticleDOI

How to Determine the Role of the Microbiome in Drug Disposition

TL;DR: Although in vitro and in vivo preclinical models are highlighted, these methods are also relevant in late-phase drug development or even as a part of routine after-market surveillance.
References
More filters
Journal ArticleDOI

limma powers differential expression analyses for RNA-sequencing and microarray studies

TL;DR: The philosophy and design of the limma package is reviewed, summarizing both new and historical features, with an emphasis on recent enhancements and features that have not been previously described.
Journal ArticleDOI

Linear Models and Empirical Bayes Methods for Assessing Differential Expression in Microarray Experiments

TL;DR: The hierarchical model of Lonnstedt and Speed (2002) is developed into a practical approach for general microarray experiments with arbitrary numbers of treatments and RNA samples and the moderated t-statistic is shown to follow a t-distribution with augmented degrees of freedom.
Journal ArticleDOI

R: A Language for Data Analysis and Graphics

TL;DR: In this article, the authors discuss their experience designing and implementing a statistical computing language, which combines what they felt were useful features from two existing computer languages, and they feel that the new language provides advantages in the areas of portability, computational efficiency, memory management, and scope.
Journal ArticleDOI

Diet rapidly and reproducibly alters the human gut microbiome

TL;DR: Increases in the abundance and activity of Bilophila wadsworthia on the animal-based diet support a link between dietary fat, bile acids and the outgrowth of microorganisms capable of triggering inflammatory bowel disease.
Journal Article

HIV-1 Entry Cofactor: Functional cDNA Cloning of a Seven-Transmembrane, G Protein–Coupled Receptor

TL;DR: Fusin this article is a putative G protein-coupled receptor with seven transmembrane segments, which enabled CD4-expressing nonhuman cell types to support HIV-1 Env-mediated cell fusion and infection.
Related Papers (5)