A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
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Under-Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle-Based Approach For Microscale Cell Culture
Chao Li,Mouhita Humayun,Glenn M. Walker,Keon Young Park,Bryce Connors,Jun Feng,Molly C. Pellitteri Hahn,Cameron O. Scarlett,Jiayi Li,Yanbo Feng,Ryan L. Clark,Hunter Hefti,Jonathan H Schrope,Ophelia S. Venturelli,David J. Beebe +14 more
TL;DR: In this paper, the authors present a method to establish and dynamically monitor autonomously regulated oxygen microenvironments (AROM) using an oil overlay in an open microscale cell culture system.
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Under-oil autonomously regulated oxygen microenvironments for cell culture
Chao Li,Mouhita Humayun,Keon Young Park,Bryce Connors,Jun Feng,Jiayi Li,Yanbo Feng,Ryan L. Clark,Hunter Hefti,Jonathan Schrope,Ophelia S. Venturelli,David J. Beebe +11 more
TL;DR: This work presents a method to establish and dynamically monitor autonomously regulated oxygen microenvironments (AROM) using an oil overlay in an open microfluidic cell culture system and experimentally validate the method using a variety of cell types including mammalian, fungal and bacterial cells.
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Applications of human organoids in the personalized treatment for digestive diseases
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Computational Biology and Machine Learning Approaches to Study Mechanistic Microbiomehost Interactions
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