A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
Citations
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The Human Gut Microbiome: From Association to Modulation
TL;DR: The type of studies that will be essential for translating microbiome research into targeted modulations with dedicated benefits for the human host are discussed.
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Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids
Magdalena Kasendra,Alessio Tovaglieri,Alessio Tovaglieri,Alexandra Sontheimer-Phelps,Alexandra Sontheimer-Phelps,Sasan Jalili-Firoozinezhad,Sasan Jalili-Firoozinezhad,Amir Bein,Angeliki Chalkiadaki,William Scholl,Cheng Zhang,Hannah Rickner,Camilla A. Richmond,Hu Li,David T. Breault,David T. Breault,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +18 more
TL;DR: The Intestine Chip may be useful as a research tool for applications where normal intestinal function is crucial, including studies of metabolism, nutrition, infection, and drug pharmacokinetics, as well as personalized medicine.
Journal ArticleDOI
A Complex Human-Gut Microbiome Cultured in an Anaerobic Intestine-on-a-Chip
Sasan Jalili-Firoozinezhad,Sasan Jalili-Firoozinezhad,Francesca S. Gazzaniga,Francesca S. Gazzaniga,Elizabeth Calamari,Diogo M. Camacho,Cicely W. Fadel,Amir Bein,Ben Swenor,Bret Nestor,Michael J. Cronce,Alessio Tovaglieri,Alessio Tovaglieri,Oren Levy,Katherine E. Gregory,Katherine E. Gregory,David T. Breault,David T. Breault,Joaquim M. S. Cabral,Dennis L. Kasper,Richard M. Novak,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +23 more
TL;DR: The extended coculture of living human intestinal epithelium with stable communities of aerobic and anaerobic human gut microbiota is shown, using a microfluidic intestine-on-a-chip that permits the control and real-time assessment of physiologically relevant oxygen gradients.
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Organs on a chip: a fast-track for engineered human tissues in drug development
TL;DR: The design considerations for single and multi-organ Oocs are reviewed, remaining challenges are discussed, and the potential impact of OOCs as a fast-track opportunity for tissue engineering to advance drug development and precision medicine is highlighted.
Journal ArticleDOI
Microfluidic Organ-on-a-Chip Models of Human Intestine.
Amir Bein,Woojung Shin,Sasan Jalili-Firoozinezhad,Sasan Jalili-Firoozinezhad,Min Hee Park,Alexandra Sontheimer-Phelps,Alexandra Sontheimer-Phelps,Alessio Tovaglieri,Alessio Tovaglieri,Angeliki Chalkiadaki,Hyun-Jung Kim,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +13 more
TL;DR: How microfluidic Intestine Chips offer new capabilities not possible with conventional culture systems or organoid cultures, including the ability to analyze contributions of individual cellular, chemical, and physical control parameters one-at-a-time is described.
References
More filters
Journal ArticleDOI
A Lactate-Induced Response to Hypoxia
Dong Chul Lee,Hyun Ahm Sohn,Zee-Yong Park,Sangho Oh,Yun Kyung Kang,Kyoung-min Lee,Minho Kang,Ye Jin Jang,Suk-Jin Yang,Young Ki Hong,Hanmi Noh,Jung-Ae Kim,Jung-Ae Kim,Dong Joon Kim,Kwang-Hee Bae,Dong Min Kim,Sang J. Chung,Hyang Sook Yoo,Dae-Yeul Yu,Kyung Chan Park,Young Il Yeom,Young Il Yeom +21 more
TL;DR: It is found that the NDRG3 protein is degraded in a PHD2/VHL-dependent manner in normoxia but is protected from destruction by binding to lactate that accumulates under hypoxia.
Journal ArticleDOI
The role of the intestinal microbiota in type 1 diabetes mellitus
Mikael Knip,Heli Siljander +1 more
TL;DR: The gut microbiota in individuals with preclinical T1DM is characterized by Bacteroidetes dominating at the phylum level, a dearth of butyrate-producing bacteria, reduced bacterial and functional diversity and low community stability, which suggests that the intestinal microbiota might be involved in the progression from β-cell autoimmunity to clinical disease rather than in the initiation of the disease process.
Journal ArticleDOI
Probiotics and gastrointestinal health.
TL;DR: Lactobacillus GG, a variant of L. casei sps rhamnosus, has been studied extensively in adults and children as mentioned in this paper, and has proven beneficial effects on intestinal immunity.
Journal ArticleDOI
Human mucosal in vivo transcriptome responses to three lactobacilli indicate how probiotics may modulate human cellular pathways
Peter van Baarlen,Freddy J. Troost,Cindy van der Meer,Guido J. E. J. Hooiveld,Mark V. Boekschoten,Robert Brummer,Michiel Kleerebezem +6 more
TL;DR: This study elucidates how intestinal mucosa of healthy humans perceives different probiotics and provides avenues for rationally designed tests of clinical applications.
Journal ArticleDOI
Oral exposure to benzo[a]pyrene in the mouse: detoxication by inducible cytochrome P450 is more important than metabolic activation.
Shigeyuki Uno,Timothy P. Dalton,Sandrine Derkenne,Christine P. Curran,Marian L. Miller,Howard G. Shertzer,Daniel W. Nebert +6 more
TL;DR: The present data indicate that, in the intact animal, inducible CYP1A1 is extremely important in detoxication and protection against oral BaP toxicity.