A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
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Synthetic ecology of the human gut microbiota.
TL;DR: This Review provides an overview of the current synthetic ecology strategies that can be used towards a more comprehensive understanding of the human gut ecosystem and presents synthetic ecology approaches that reduce the complexity and advance translation of human gut microbiota research.
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Gut-on-a-chip: Current progress and future opportunities.
Nureddin Ashammakhi,Rohollah Nasiri,Natan Roberto de Barros,Peyton Tebon,Jai Thakor,Marcus J. Goudie,Amir Shamloo,Martin G. Martin,Ali Khademhosseini +8 more
TL;DR: Gut-on-a-chip models have the potential to advance the understanding of the basic interactions found within the gut and lay the foundation for future applications in understanding pathophysiology, developing drugs, and personalizing medical treatments.
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A Robust Longitudinal Co-culture of Obligate Anaerobic Gut Microbiome With Human Intestinal Epithelium in an Anoxic-Oxic Interface-on-a-Chip.
Woojung Shin,Alexander Wu,Miles W. Massidda,Charles R. Foster,Newin Thomas,Dong Woo Lee,Hong Koh,Youngwon Ju,Joohoon Kim,Hyun-Jung Kim,Hyun-Jung Kim +10 more
TL;DR: This new protocol for creating an AOI in a microfluidic gut-on-a-chip may enable to demonstrate the key physiological interactions of obligate anaerobic gut microbiome with the host cells associated with intestinal metabolism, homeostasis, and immune regulation.
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An end-user perspective on Organ-on-a-Chip : Assays and usability aspects
TL;DR: It is concluded that the elements that enable the leap towards end-user adoption are in place, but only few systems have managed to incorporate all aspects, and are able to answer biological questions.
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Dark matter in host-microbiome metabolomics: Tackling the unknowns-A review.
TL;DR: A close look is taken at developments in the mutli-omic analyses and the use of mass spectrometry to investigate the exchange of metabolites between the host and the microbiome as well as the environment within the microbiome.
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