A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
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Development of an in vitro Model of Human Gut Microbiota for Screening the Reciprocal Interactions With Antibiotics, Drugs, and Xenobiotics
Abdelaziz El Houari,Florine Ecale,Anne Mercier,Stéphanie Crapart,Jérôme Laparre,Baptiste Soulard,Manilduth Ramnath,Jean-Marc Berjeaud,Marie-Hélène Rodier,Alexandre Crépin +9 more
TL;DR: The results suggest that biotransformation of molecules occurred in the presence of the gut microbiota model and the coupled approaches performed on the individual cultures may emphasize new bacterial strains active in these metabolic processes.
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Organ-on-Chip Technology for Aerobic Intestinal Host – Anaerobic Microbiota Research
TL;DR: A review of AHAM-on-chip systems can be found in this article , where the authors summarize existing models for AHAM interfaces and provide an overview of four different AHAM on-chip system components.
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Organs-on-Chips Platforms Are Everywhere: A Zoom on Biomedical Investigation
Mohamed Zommiti,Nathalie Connil,Ali Tahrioui,A. Groboillot,Corinne Barbey,Yoan Konto-Ghiorghi,Olivier Lesouhaitier,Sylvie Chevalier,Marc G. J. Feuilloley +8 more
TL;DR: Organ-on-a-chip (OOC) is a state-of-the-art microfluidic cell culture technology that sums up cells or tissue-to-tissue interfaces, fluid flows, mechanical cues, and organ-level physiology, and it has been developed to fill the gap between in vitro experimental models and human pathophysiology as discussed by the authors .
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Microfluidic Gut-on-a-Chip: Fundamentals and Challenges
TL;DR: GOC has become a popular substitute for animal models, which is capable of imitating complex systems in vitro and has been used to study pathology and pharmacology as mentioned in this paper , but the challenges and solutions to these limitations were often overlooked.
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The role of probiotics on the roadmap to a healthy microbiota: a symposium report
TL;DR: The ninth International Yakult Symposium was held in Ghent, Belgium in April 2018 and included talks from Professor Wijmenga on using biobanks to understand the relationship between the gut microbiota and health; and Professor Hill on phage–probiotic interactions.
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