A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
Citations
More filters
Journal ArticleDOI
The Human Gut Microbiome: From Association to Modulation
TL;DR: The type of studies that will be essential for translating microbiome research into targeted modulations with dedicated benefits for the human host are discussed.
Journal ArticleDOI
Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids
Magdalena Kasendra,Alessio Tovaglieri,Alessio Tovaglieri,Alexandra Sontheimer-Phelps,Alexandra Sontheimer-Phelps,Sasan Jalili-Firoozinezhad,Sasan Jalili-Firoozinezhad,Amir Bein,Angeliki Chalkiadaki,William Scholl,Cheng Zhang,Hannah Rickner,Camilla A. Richmond,Hu Li,David T. Breault,David T. Breault,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +18 more
TL;DR: The Intestine Chip may be useful as a research tool for applications where normal intestinal function is crucial, including studies of metabolism, nutrition, infection, and drug pharmacokinetics, as well as personalized medicine.
Journal ArticleDOI
A Complex Human-Gut Microbiome Cultured in an Anaerobic Intestine-on-a-Chip
Sasan Jalili-Firoozinezhad,Sasan Jalili-Firoozinezhad,Francesca S. Gazzaniga,Francesca S. Gazzaniga,Elizabeth Calamari,Diogo M. Camacho,Cicely W. Fadel,Amir Bein,Ben Swenor,Bret Nestor,Michael J. Cronce,Alessio Tovaglieri,Alessio Tovaglieri,Oren Levy,Katherine E. Gregory,Katherine E. Gregory,David T. Breault,David T. Breault,Joaquim M. S. Cabral,Dennis L. Kasper,Richard M. Novak,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +23 more
TL;DR: The extended coculture of living human intestinal epithelium with stable communities of aerobic and anaerobic human gut microbiota is shown, using a microfluidic intestine-on-a-chip that permits the control and real-time assessment of physiologically relevant oxygen gradients.
Journal ArticleDOI
Organs on a chip: a fast-track for engineered human tissues in drug development
TL;DR: The design considerations for single and multi-organ Oocs are reviewed, remaining challenges are discussed, and the potential impact of OOCs as a fast-track opportunity for tissue engineering to advance drug development and precision medicine is highlighted.
Journal ArticleDOI
Microfluidic Organ-on-a-Chip Models of Human Intestine.
Amir Bein,Woojung Shin,Sasan Jalili-Firoozinezhad,Sasan Jalili-Firoozinezhad,Min Hee Park,Alexandra Sontheimer-Phelps,Alexandra Sontheimer-Phelps,Alessio Tovaglieri,Alessio Tovaglieri,Angeliki Chalkiadaki,Hyun-Jung Kim,Donald E. Ingber,Donald E. Ingber,Donald E. Ingber +13 more
TL;DR: How microfluidic Intestine Chips offer new capabilities not possible with conventional culture systems or organoid cultures, including the ability to analyze contributions of individual cellular, chemical, and physical control parameters one-at-a-time is described.
References
More filters
Journal ArticleDOI
Reappraisal of the Immunophenotype of Pancreatic Intraductal Papillary Mucinous Neoplasms (IPMNs)—Gastric Pyloric and Small Intestinal Immunophenotype Expression in Gastric and Intestinal Type IPMNs—
TL;DR: Gastric pyloric and small intestinal differentiation are characteristic of gastric and intestinal type IPMN, respectively, and these two IPMN types may have distinct pathogenesis.
Journal ArticleDOI
NDRG3-mediated lactate signaling in hypoxia.
TL;DR: The NDRG3-Raf-ERK axis provides the genetic basis for lactate-inducedhypoxia signaling, which can be exploited for the development of therapies targeting hypoxia-induced diseases in addition to advancing the understanding of the normal physiology of Hypoxia responses.
Journal Article
Gene structure and regulation of expression of human glutathione S-transferases alpha.
TL;DR: The induction of human GSTs by drugs or nutritional constituents would justify an interest for developing chemointervention strategies in populations highly exposed to carcinogens like aflatoxin B1 and polycyclic aromatic hydrocarbons.
Journal ArticleDOI
In vivo gut transcriptome responses to Lactobacillus rhamnosus GG and Lactobacillus acidophilus in neonatal gnotobiotic piglets.
Anand Kumar,Anastasia N. Vlasova,Zhe Liu,Kuldeep S. Chattha,Sukumar Kandasamy,Malak A. Esseili,Xiaoli Zhang,Gireesh Rajashekara,Linda J. Saif +8 more
TL;DR: Data indicated that probiotic establishment and beneficial effects in the host are guided by down-regulation or upregulation of immune function-related genes in the early and later stages of colonization, respectively, and alternations in metabolism of small molecules (vitamins and/or minerals) and macromolecules (carbohydrates, proteins, and lipids).
Journal ArticleDOI
Elevated C-peptide and insulin predict increased risk of colorectal adenomas in normal mucosa.
Adriana C. Vidal,Pauline K. Lund,Cathrine Hoyo,Joseph A. Galanko,Lauren Burcal,Rachel Holston,Berri Massa,Oluwaseun Omofoye,Robert S. Sandler,Temitope O. Keku +9 more
TL;DR: Associations between these peptides and the apoptosis index in overweight and obese individuals suggest that the mechanism by which C-peptide could induce adenomas may include its anti-apoptotic properties.