A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Pranjul Shah,Joëlle V. Fritz,Enrico Glaab,Mahesh Desai,Kacy Greenhalgh,Audrey Frachet,Magdalena Niegowska,Matthew Estes,Christian Jäger,Carole Seguin-Devaux,Frederic Zenhausern,Paul Wilmes +11 more
TLDR
The ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions is demonstrated.Abstract:
We thank the scientists and technical staff of the Luxembourg Centre for Systems Biomedicine and Center for Applied Nanobioscience and Medicine, particularly Matthew Barrett and Brett Duane for their excellent technical assistance and engineering support We are grateful to Francois Bernardin, Nathalie Nicot and Laurent Vallar for the microarray analysis; Aidos Baumuratov for imaging support; Linda Wampach for HuMiX illustrations; and Anna Heintz-Buschart for fruitful discussions This work was supported by an ATTRACT programme grant (ATTRACT/A09/03), a CORE programme grant (CORE/11/BM/1186762), a European Union Joint Programming in Neurodegenerative Diseases grant (INTER/JPND/12/01) and a Proof-of-Concept grant (PoC-15/11014639) to PW, Accompany Measures mobility grant (12/AM2c/05) to PW and PS, an INTER mobility grant to PS (INTER/14/7516918), and an Aide a la Formation Recherche (AFR) postdoctoral grant (AFR/PDR 2013-1/BM/5821107) as well as a CORE programme grant (CORE/14/BM/8066232) to JVF, all funded by the Luxembourg National Research Fund (FNR) This work was further supported by a grant attributed to CS-D by the 'Fondation Recherche sur le SIDA du Luxembourg' Bioinformatics analyses presented in this paper were carried out in part using the HPC facilities of the University of Luxembourg (http://hpcunilu)read more
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Development of a novel human intestinal model to elucidate the effect of anaerobic commensals on Escherichia coli infection
Conor J. McGrath,Edgaras Laveckis,Andrew Harwood Bell,Emmanuelle H. Crost,Nathalie Juge,Stephanie Schüller +5 more
TL;DR: The development of a microaerobic, mucus-producing vertical diffusion chamber (VDC) model will facilitate investigations into the mechanisms underpinning colonization resistance and aid the development of microbiota-based anti-infection strategies.
Posted ContentDOI
Metabolomics and proteomics of L. rhamnosus GG and E. coli Nissle probiotic supernatants identify distinct pathways that mediate growth suppression of antimicrobial-resistant pathogens
Petronella R. Hove,Nora Jean Nealon,Siu Hung Joshua Chan,Shea M. Boyer,Hannah B. Haberecht,Elizabeth P. Ryan +5 more
TL;DR: In this article, the performance of two probiotic molecules, Gram-positive L. rhamnosus GG (LGG) and Gram-negative E. coli Nissle (ECN), was evaluated in a dose-dependent manner for differential growth suppression of Salmonella Typhimurium, Escherichia coli, and Klebsiella oxytoca that harbor antimicrobial resistance (AMR).
Posted ContentDOI
Innate immune cell response to host-parasite interaction in a human intestinal tissue microphysiological system
Mouhita Humayun,Jose M. Ayuso,Keon Young Park,Bruno Martorelli Di Genova,Melissa C. Skala,Sheena C. Kerr,Laura J. Knoll,David Beebe +7 more
TL;DR: A human microphysiological system of intestinal tissue is developed to evaluate parasite-immune-specific interactions during infection, which integrates primary intestinal epithelial cells and immune cells to investigate the role of innate immune cells during epithelial infection by the protozoan parasite, Toxoplasma gondii.
Journal ArticleDOI
Development of a Pumpless Microfluidic System to Study the Interaction between Gut Microbes and Intestinal Epithelial Cells
Seung Yeon Lee,Hyun Jae Byun,Heelak Choi,Jong-In Won,Jeonghun Han,Sungsu Park,Donghyun Kim,Jong Hwan Sung +7 more
TL;DR: A novel gut microbialepithelial cell (GMEC) co-culture system, a microfluidic chip in an anaerobic chamber with oxygen gradient within the chip, is developed that can potentially be a useful platform for studying the interaction between intestinal epithelial cells and gut microbes.
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