An insect-specific P450 oxidative decarbonylase for cuticular hydrocarbon biosynthesis
Yue Qiu,Claus Tittiger,Claude Wicker-Thomas,Gaëlle Le Goff,Sharon Young,Eric Wajnberg,Thierry Fricaux,Nathalie Taquet,Gary J. Blomquist,René Feyereisen +9 more
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TLDR
It is shown that insects use a P450 enzyme of the CYP4G family to oxidatively produce hydrocarbons from aldehydes with the release of carbon dioxide, showing that the insect enzyme is an oxidative decarbonylase that catalyzes the cleavage of long-chain alde Hydrocarbon biosynthesis from long- chain fatty aldeHydes.Abstract:
Insects use hydrocarbons as cuticular waterproofing agents and as contact pheromones. Although their biosynthesis from fatty acyl precursors is well established, the last step of hydrocarbon biosynthesis from long-chain fatty aldehydes has remained mysterious. We show here that insects use a P450 enzyme of the CYP4G family to oxidatively produce hydrocarbons from aldehydes. Oenocyte-directed RNAi knock-down of Drosophila CYP4G1 or NADPH-cytochrome P450 reductase results in flies deficient in cuticular hydrocarbons, highly susceptible to desiccation, and with reduced viability upon adult emergence. The heterologously expressed enzyme converts C18-trideuterated octadecanal to C17-trideuterated heptadecane, showing that the insect enzyme is an oxidative decarbonylase that catalyzes the cleavage of long-chain aldehydes to hydrocarbons with the release of carbon dioxide. This process is unlike cyanobacteria that use a nonheme diiron decarbonylase to make alkanes from aldehydes with the release of formate. The unique and highly conserved insect CYP4G enzymes are a key evolutionary innovation that allowed their colonization of land.read more
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Transformation of biomass into commodity chemicals using enzymes or cells
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Arabidopsis cuticular waxes: advances in synthesis, export and regulation.
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The genomes of two key bumblebee species with primitive eusocial organization
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Genomic signatures of evolutionary transitions from solitary to group living
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An algal photoenzyme converts fatty acids to hydrocarbons
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