An optimized acetylcholine sensor for monitoring in vivo cholinergic activity.
Miao Jing,Li Y,Li Y,Jianzhi Zeng,Jianzhi Zeng,Pengcheng Huang,Miguel Skirzewski,Ornela Kljakic,Wanling Peng,Tongrui Qian,Tongrui Qian,Ke Tan,Ke Tan,Jing Zou,Simon Trinh,Wu Runlong,Shichen Zhang,Shichen Zhang,Sunlei Pan,Sunlei Pan,Samuel Andrew Hires,Min Xu,Haohong Li,Lisa M. Saksida,Vania F. Prado,Timothy J. Bussey,Marco A. M. Prado,Liangyi Chen,Heping Cheng,Heping Cheng,Yulong Li,Yulong Li +31 more
Reads0
Chats0
TLDR
The GRAB ACh (GPCR-activation-based ACh) sensor is optimized to achieve substantially improved sensitivity in ACh detection, as well as reduced downstream coupling to intracellular pathways.Abstract:
The ability to directly measure acetylcholine (ACh) release is an essential step toward understanding its physiological function. Here we optimized the GRABACh (GPCR-activation-based ACh) sensor to achieve substantially improved sensitivity in ACh detection, as well as reduced downstream coupling to intracellular pathways. The improved version of the ACh sensor retains the subsecond response kinetics, physiologically relevant affinity and precise molecular specificity for ACh of its predecessor. Using this sensor, we revealed compartmental ACh signals in the olfactory center of transgenic flies in response to external stimuli including odor and body shock. Using fiber photometry recording and two-photon imaging, our ACh sensor also enabled sensitive detection of single-trial ACh dynamics in multiple brain regions in mice performing a variety of behaviors.read more
Citations
More filters
Journal ArticleDOI
Next-generation GRAB sensors for monitoring dopaminergic activity in vivo.
Fangmiao Sun,Fangmiao Sun,Jingheng Zhou,Bing Dai,Tongrui Qian,Tongrui Qian,Jianzhi Zeng,Jianzhi Zeng,Jianzhi Zeng,Xuelin Li,Xuelin Li,Yizhou Zhuo,Yizhou Zhuo,Yizhou Zhuo,Yajun Zhang,Yajun Zhang,Yajun Zhang,Yipan Wang,Yipan Wang,Cheng Qian,Cheng Qian,Cheng Qian,Ke Tan,Ke Tan,Jiesi Feng,Jiesi Feng,Jiesi Feng,Hui Dong,Hui Dong,Dayu Lin,Guohong Cui,Yulong Li,Yulong Li,Yulong Li +33 more
TL;DR: The GRABDA sensors resolve evoked DA release in mouse brain slices, detect evoked compartmental DA release from a single neuron in live flies, and report optogenetically elicited nigrostriatal DA release as well as mesoaccumbens dopaminergic activity during sexual behavior in freely behaving mice.
Journal ArticleDOI
Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning.
Elizabeth K. Unger,Jacob P. Keller,Michael Altermatt,Ruqiang Liang,Aya Matsui,Chunyang Dong,Olivia J Hon,Zi Yao,Junqing Sun,Samba Banala,Meghan E. Flanigan,David A. Jaffe,Samantha Hartanto,Jane Carlen,Grace O. Mizuno,Phillip M. Borden,Amol V. Shivange,Lindsay P. Cameron,Steffen Sinning,Suzanne M. Underhill,David E. Olson,Susan G. Amara,Duncan Temple Lang,Gary Rudnick,Jonathan S. Marvin,Luke D. Lavis,Henry A. Lester,Veronica A. Alvarez,Andrew J. Fisher,Jennifer A. Prescher,Thomas L. Kash,Vladimir Yarov-Yarovoy,Viviana Gradinaru,Loren L. Looger,Lin Tian +34 more
TL;DR: iSeroSnFR can be used to detect serotonin release in freely behaving mice during fear conditioning, social interaction, and sleep/wake transitions, and it is expected that both machine-learning-guided binding-pocket redesign and iSero SnFR will have broad utility for the development of other sensors and in vitro and in-vivo serotonin detection, respectively.
Journal ArticleDOI
An action potential initiation mechanism in distal axons for the control of dopamine release
Changliang Liu,Xintong Cai,Andreas Ritzau-Jost,Paul F Kramer,Yulong Li,Zayd M. Khaliq,Stefan Hallermann,Pascal S. Kaeser +7 more
TL;DR: The findings uncover an endogenous mechanism for action potential initiation independent of somatodendritic integration and establish that this mechanism segregates the control of dopamine signaling between axons and somata.
Journal ArticleDOI
A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo
Ao Dong,Ao Dong,Kaikai He,Kaikai He,Barna Dudok,Jordan S. Farrell,Wuqiang Guan,Daniel J. Liput,Henry L. Puhl,Ruyi Cai,Ruyi Cai,Huan Wang,Huan Wang,Jiali Duan,Jiali Duan,Eddy Albarran,Jun B. Ding,David M. Lovinger,Bo Li,Ivan Soltesz,Yulong Li +20 more
TL;DR: GRABeCB2.0 as discussed by the authors consists of a circular-permutated EGFP and the human CB1 cannabinoid receptor, providing cell membrane trafficking, second-resolution kinetics with high specificity for eCBs, and shows a robust fluorescence response at physiological eCB concentrations.
Journal ArticleDOI
Characterizing Cortex-Wide Dynamics with Wide-Field Calcium Imaging.
Chi Ren,Takaki Komiyama +1 more
TL;DR: Wide-field calcium imaging, combined with improved genetically encoded calcium indicators, allows sufficient signal-to-noise ratio and spatiotemporal resolution to afford a unique opportunity to capture cortex-wide dynamics on a moment-by-moment basis in behaving animals as mentioned in this paper.
References
More filters
Journal ArticleDOI
Enzymatic assembly of DNA molecules up to several hundred kilobases
Daniel G. Gibson,Lei Young,Ray-Yuan Chuang,J. Craig Venter,Clyde A. Hutchison,Hamilton O. Smith +5 more
TL;DR: An isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a DNA ligase is described.
Journal ArticleDOI
Independent optical excitation of distinct neural populations
Nathan C. Klapoetke,Yasunobu Murata,Sung Soo Kim,Stefan R. Pulver,Amanda Birdsey-Benson,Yong Ku Cho,Tania K. Morimoto,Amy S. Chuong,Eric J. Carpenter,Zhijian Tian,Jun Wang,Yinlong Xie,Zhixiang Yan,Yong Zhang,Brian Y. Chow,Barbara Surek,Michael Melkonian,Vivek Jayaraman,Martha Constantine-Paton,Gane Ka-Shu Wong,Edward S. Boyden +20 more
TL;DR: Two channelrhodopsins, Chronos and Chrimson, are described, discovered through sequencing and physiological characterization of opsins from over 100 species of alga, that enable two-color activation of neural spiking and downstream synaptic transmission in independent neural populations without detectable cross-talk in mouse brain slice.
Journal ArticleDOI
The role of acetylcholine in learning and memory.
TL;DR: The effects in entorhinal and perirhinal cortex and hippocampus might be important for encoding new episodic memories, and computational modeling links the function of cholinergic modulation to specific cellular effects within these regions.
Independent optical excitation of distinct neural populations
Nathan C. Klapoetke,Yasunobu Murata,Sung Soo Kim,Stefan R. Pulver,Amanda Birdsey-Benson,Yong Ku Cho,Tania K. Morimoto,Amy S. Chuong,Eric J. Carpenter,Zhijian Tian,Jun Wang,Yinlong Xie,Zhixiang Yan,Yong Zhang,Brian Y. Chow,Barbara Surek,Michael Melkonian,Vivek Jayaraman,Martha Constantine-Paton,Gane Ka-Shu Wong,Edward S. Boyden +20 more
TL;DR: Chronos and Chrimson as mentioned in this paper have been shown to enable two-color activation of neural spiking and downstream synaptic transmission in independent neural populations without detectable cross-talk in mouse brain slice.
Journal ArticleDOI
Acetylcholine as a neuromodulator: cholinergic signaling shapes nervous system function and behavior
TL;DR: Action of cholinergic signaling on cellular and synaptic properties of neurons in several brain areas are identified and consequences of this signaling on behaviors related to drug abuse, attention, food intake, and affect are discussed.
Related Papers (5)
A Genetically Encoded Fluorescent Sensor Enables Rapid and Specific Detection of Dopamine in Flies, Fish, and Mice.
Fangmiao Sun,Jianzhi Zeng,Miao Jing,Miao Jing,Miao Jing,Jingheng Zhou,Jiesi Feng,Jiesi Feng,Jiesi Feng,Scott F. Owen,Yichen Luo,Funing Li,Huan Wang,Huan Wang,Takashi Yamaguchi,Zihao Yong,Yijing Gao,Wanling Peng,Lizhao Wang,Siyu Zhang,Jiulin Du,Dayu Lin,Min Xu,Anatol C. Kreitzer,Guohong Cui,Yulong Li,Yulong Li,Yulong Li +27 more