Independent optical excitation of distinct neural populations
Nathan C. Klapoetke,Yasunobu Murata,Sung Soo Kim,Stefan R. Pulver,Amanda Birdsey-Benson,Yong Ku Cho,Tania K. Morimoto,Amy S. Chuong,Eric J. Carpenter,Zhijian Tian,Jun Wang,Yinlong Xie,Zhixiang Yan,Yong Zhang,Brian Y. Chow,Barbara Surek,Michael Melkonian,Vivek Jayaraman,Martha Constantine-Paton,Gane Ka-Shu Wong,Edward S. Boyden +20 more
Reads0
Chats0
TLDR
Two channelrhodopsins, Chronos and Chrimson, are described, discovered through sequencing and physiological characterization of opsins from over 100 species of alga, that enable two-color activation of neural spiking and downstream synaptic transmission in independent neural populations without detectable cross-talk in mouse brain slice.Abstract:
Optogenetic tools enable examination of how specific cell types contribute to brain circuit functions. A long-standing question is whether it is possible to independently activate two distinct neural populations in mammalian brain tissue. Such a capability would enable the study of how different synapses or pathways interact to encode information in the brain. Here we describe two channelrhodopsins, Chronos and Chrimson, discovered through sequencing and physiological characterization of opsins from over 100 species of alga. Chrimson's excitation spectrum is red shifted by 45 nm relative to previous channelrhodopsins and can enable experiments in which red light is preferred. We show minimal visual system-mediated behavioral interference when using Chrimson in neurobehavioral studies in Drosophila melanogaster. Chronos has faster kinetics than previous channelrhodopsins yet is effectively more light sensitive. Together these two reagents enable two-color activation of neural spiking and downstream synaptic transmission in independent neural populations without detectable cross-talk in mouse brain slice.read more
Citations
More filters
Journal ArticleDOI
Near-infrared deep brain stimulation via upconversion nanoparticle–mediated optogenetics
Shuo Chen,Adam Z. Weitemier,Xiao Zeng,Linmeng He,Xiyu Wang,Yanqiu Tao,Arthur J Huang,Yuki Hashimotodani,Masanobu Kano,Hirohide Iwasaki,Laxmi Kumar Parajuli,Shigeo Okabe,Daniel Boon Loong Teh,Angelo H. All,Iku Tsutsui-Kimura,Kenji F. Tanaka,Xiaogang Liu,Xiaogang Liu,Thomas J. McHugh,Thomas J. McHugh +19 more
TL;DR: It is demonstrated that molecularly tailored UCNPs can serve as optogenetic actuators of transcranial NIR light to stimulate deep brain neurons and evoked dopamine release from genetically tagged neurons in the ventral tegmental area and triggered memory recall.
Journal ArticleDOI
All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
Daniel Hochbaum,Yongxin Zhao,Samouil L. Farhi,Nathan C. Klapoetke,Christopher A. Werley,Vikrant Kapoor,Peng Zou,Joel M. Kralj,Dougal Maclaurin,Niklas Smedemark-Margulies,Jessica L. Saulnier,Gabriella L. Boulting,Christoph Straub,Yong Ku Cho,Michael Melkonian,Gane Ka-Shu Wong,D. Jed Harrison,Venkatesh N. Murthy,Bernardo L. Sabatini,Edward S. Boyden,Robert E. Campbell,Adam E. Cohen +21 more
TL;DR: In this article, a coexpression vector, Optopatch, enabled cross-talk-free genetically targeted all-optical electrophysiology without the use of conventional electrodes.
Journal ArticleDOI
Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila
Yoshinori Aso,Divya Sitaraman,Toshiharu Ichinose,Karla R. Kaun,Katrin Vogt,Ghislain Belliart-Guérin,Pierre-Yves Plaçais,Alice A. Robie,Nobuhiro Yamagata,Christopher Schnaitmann,William J Rowell,Rebecca M. Johnston,Teri T.B. Ngo,Nan Chen,Wyatt Korff,Michael N. Nitabach,Ulrike Heberlein,Thomas Preat,Kristin Branson,Hiromu Tanimoto,Gerald M. Rubin +20 more
TL;DR: This work studied the role of MBONs in several associative learning tasks and in sleep regulation, revealing the extent to which information flow is segregated into distinct channels and suggesting possible roles for the multi-layered MBON network.
Journal ArticleDOI
Data access for the 1,000 Plants (1KP) project.
Naim Matasci,Ling-Hong Hung,Zhixiang Yan,Eric J. Carpenter,Norman J. Wickett,Siavash Mirarab,Nam Nguyen,Tandy Warnow,Saravanaraj Ayyampalayam,Michael S. Barker,J. G. Burleigh,Matthew A. Gitzendanner,Eric K. Wafula,Joshua P. Der,Claude W. dePamphilis,Béatrice Roure,Hervé Philippe,Hervé Philippe,Brad R. Ruhfel,Brad R. Ruhfel,Nicholas W. Miles,Sean W. Graham,Sarah Mathews,Barbara Surek,Michael Melkonian,Douglas E. Soltis,Pamela S. Soltis,Carl J. Rothfels,Carl J. Rothfels,Lisa Pokorny,Lisa Pokorny,Jonathan Shaw,Lisa DeGironimo,Dennis W. Stevenson,Juan Carlos Villarreal,Tao Chen,Toni M. Kutchan,Megan Rolf,Regina S. Baucom,Michael K. Deyholos,Ram Samudrala,Zhijian Tian,Xiaolei Wu,Xiao Sun,Yong Zhang,Jun Wang,Jim Leebens-Mack,Gane Ka-Shu Wong,Gane Ka-Shu Wong +48 more
TL;DR: How to access the data used in a phylogenomics analysis of the first 85 species, and how to visualize the gene and species trees of the 1KP project.
Journal ArticleDOI
Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics
TL;DR: Anion channel rhodopsins (ACRs), a family of light-gated anion channels from cryptophyte algae that provide highly sensitive and efficient membrane hyperpolarization and neuronal silencing through light- gated chloride conduction, are described.
References
More filters
Journal ArticleDOI
Millisecond-timescale, genetically targeted optical control of neural activity.
TL;DR: In this paper, the authors adapted the naturally occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel, by using lentiviral gene delivery in combination with high-speed optical switching to photostimulate mammalian neurons.
Journal ArticleDOI
Neocortical excitation/inhibition balance in information processing and social dysfunction
Ofer Yizhar,Ofer Yizhar,Lief E. Fenno,Matthias Prigge,Franziska Schneider,Thomas J. Davidson,Daniel J. O’Shea,Vikaas S. Sohal,Vikaas S. Sohal,Inbal Goshen,Joel Finkelstein,Jeanne T. Paz,Katja Stehfest,Roman Fudim,Charu Ramakrishnan,John R. Huguenard,Peter Hegemann,Karl Deisseroth +17 more
TL;DR: In this article, the authors design and use several optogenetic tools to causally investigate the cellular E/I balance hypothesis in freely moving mammals, and explore the associated circuit physiology.
Journal ArticleDOI
Multimodal fast optical interrogation of neural circuitry
Feng Zhang,Liping Wang,Martin Brauner,Jana F. Liewald,Kenneth Kay,Natalie Watzke,Phillip G. Wood,Ernst Bamberg,Ernst Bamberg,Georg Nagel,Alexander Gottschalk,Karl Deisseroth +11 more
TL;DR: An archaeal light-driven chloride pump from Natronomonas pharaonis is identified and developed for temporally precise optical inhibition of neural activity and forms a complete system for multimodal, high-speed, genetically targeted, all-optical interrogation of living neural circuits.
Journal ArticleDOI
High-performance genetically targetable optical neural silencing by light-driven proton pumps
Brian Y. Chow,Xue Han,Xue Han,Allison S. Dobry,Allison S. Dobry,Xiaofeng Qian,Xiaofeng Qian,Amy S. Chuong,Amy S. Chuong,Mingjie Li,Mingjie Li,Henninger Michael,Henninger Michael,Gabriel M. Belfort,Yingxi Lin,Patrick E. Monahan,Patrick E. Monahan,Edward S. Boyden,Edward S. Boyden +18 more
TL;DR: Light-driven proton pumps represent a high-performance and extremely versatile class of ‘optogenetic’ voltage and ion modulator, which will broadly enable new neuroscientific, biological, neurological and psychiatric investigations.
Journal ArticleDOI
Refinement of Tools for Targeted Gene Expression in Drosophila
Barret D. Pfeiffer,Teri-T B. Ngo,Karen L. Hibbard,Christine Murphy,Arnim Jenett,James W Truman,Gerald M. Rubin +6 more
TL;DR: The increased strength and reliability of these optimized reagents overcome many of the previous limitations of these methods and will facilitate genetic manipulations of greater complexity and sophistication in Drosophila melanogaster.
Related Papers (5)
A GAL4-Driver Line Resource for Drosophila Neurobiology
Arnim Jenett,Gerald M. Rubin,Teri-T B. Ngo,David Shepherd,Christine Murphy,Heather Dionne,Barret D. Pfeiffer,Amanda Cavallaro,Donald Hall,Jennifer Jeter,Nirmala Iyer,Dona Fetter,Joanna H. Hausenfluck,Hanchuan Peng,Eric T. Trautman,Robert Svirskas,Eugene W. Myers,Zbigniew R. Iwinski,Yoshinori Aso,Gina M. DePasquale,Adrianne Enos,Phuson Hulamm,S. Lam,Hsing-Hsi Li,Todd R. Laverty,Fuhui Long,Lei Qu,Sean D. Murphy,Konrad Rokicki,Todd Safford,Kshiti Shaw,Julie H. Simpson,Allison Sowell,Susana Tae,Yang Yu,Christopher T. Zugates +35 more