Journal ArticleDOI
Detecting biomarkers with microdroplet technology
TLDR
Digital procedures based on the limiting dilution of biological samples in individual compartments such as droplets of a water-in-oil emulsion, and relies on the discrete counting of a given event, providing an absolute value and quantitative data could become an essential diagnostic tool for the study of diseases as well as patient management.About:
This article is published in Trends in Molecular Medicine.The article was published on 2012-07-01. It has received 110 citations till now.read more
Citations
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Journal ArticleDOI
Single-cell analysis and sorting using droplet-based microfluidics
Linas Mazutis,John R. Gilbert,W. Lloyd Ung,David A. Weitz,Andrew D. Griffiths,John A. Heyman +5 more
TL;DR: A droplet-based microfluidics protocol for high-throughput analysis and sorting of single cells, and a binding assay for detecting antibodies secreted from single mouse hybridoma cells is detailed.
Journal ArticleDOI
Mutation Tracking in Circulating Tumor DNA Predicts Relapse in Early Breast Cancer
Isaac Garcia-Murillas,Gaia Schiavon,Gaia Schiavon,Britta Weigelt,Charlotte K.Y. Ng,Sarah Hrebien,Rosalind J. Cutts,Maggie C.U. Cheang,Peter Osin,Ashutosh Nerurkar,Iwanka Kozarewa,Javier Armisen Garrido,Mitch Dowsett,Mitch Dowsett,Jorge S. Reis-Filho,Ian E. Smith,Nicholas C. Turner,Nicholas C. Turner +17 more
TL;DR: In a prospective cohort of 55 early breast cancer patients receiving neoadjuvant chemotherapy, detection of ctDNA in plasma after completion of apparently curative treatment—either at a single postsurgical time point or with serial follow-up plasma samples—predicted metastatic relapse with high accuracy and increased sensitivity.
Journal ArticleDOI
Multiplex Picodroplet Digital PCR to Detect KRAS Mutations in Circulating DNA from the Plasma of Colorectal Cancer Patients
Valérie Taly,Deniz Pekin,Deniz Pekin,Leonor Benhaim,Steve K. Kotsopoulos,Delphine Le Corre,Xinyu Li,Ivan Atochin,Darren R. Link,Andrew D. Griffiths,Andrew D. Griffiths,Karine Pallier,Hélène Blons,Olivier Bouché,Bruno Landi,J. Brian Hutchison,Pierre Laurent-Puig +16 more
TL;DR: Clinical utility of multiplex dPCR to screen for multiple mutations simultaneously with a sensitivity sufficient to detect mutations in circulating DNA obtained by noninvasive blood collection is demonstrated.
Journal ArticleDOI
High-Throughput Genomic Data in Systematics and Phylogenetics
TL;DR: This review presents recent advances in laboratory methods for collection of high-throughput phylogenetic data and challenges and constraints for phylogenetic analysis of these data, and offers recommendations for the most promising protocols and data-analysis workflows currently available.
Journal ArticleDOI
dPCR: A Technology Review.
TL;DR: This review compares the fundamental concepts behind the quantification of nucleic acids by dPCR and quantitative real-time PCR and examines how isothermal amplification could be an alternative to PCR in digital assays.
References
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Genome sequencing in microfabricated high-density picolitre reactors
Marcel Margulies,Michael Egholm,William E. Altman,Said Attiya,Joel S. Bader,Lisa A. Bemben,Jan Berka,Michael S. Braverman,Yi-Ju Chen,Zhoutao Chen,Scott Dewell,Lei Du,J. M. Fierro,Xavier V. Gomes,Brian C. Godwin,Wen He,Scott Edward Helgesen,Chun Heen Ho,Gerard P. Irzyk,Szilveszter C. Jando,Maria L. I. Alenquer,Thomas P. Jarvie,Kshama B. Jirage,Jong-Bum Kim,James R. Knight,Janna R. Lanza,John H. Leamon,Steven Lefkowitz,Ming Lei,Jing Li,Kenton Lohman,Hong Lu,Vinod Makhijani,Keith Mcdade,Michael P. McKenna,Eugene W. Myers,Elizabeth Nickerson,John Nobile,Ramona Plant,Bernard P. Puc,Michael T. Ronan,George T. Roth,Gary J. Sarkis,Jan Fredrik Simons,John Simpson,Maithreyan Srinivasan,Karrie R. Tartaro,Alexander Tomasz,Kari A. Vogt,Greg A. Volkmer,Shally H. Wang,Yong Wang,Michael P. Weiner,Pengguang Yu,Richard F. Begley,Jonathan M. Rothberg +55 more
TL;DR: A scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments with 96% coverage at 99.96% accuracy in one run of the machine is described.
Journal ArticleDOI
Sequencing technologies-the next generation
TL;DR: A technical review of template preparation, sequencing and imaging, genome alignment and assembly approaches, and recent advances in current and near-term commercially available NGS instruments is presented.
Journal ArticleDOI
An integrated semiconductor device enabling non-optical genome sequencing
Jonathan M. Rothberg,Wolfgang Hinz,Todd Rearick,Jonathan Schultz,William J. Mileski,Melville Davey,John H. Leamon,Kim L. Johnson,Mark James Milgrew,Matthew D. Edwards,Jeremy Hoon,Jan Fredrik Simons,David Marran,Jason W. Myers,John F. Davidson,Annika Branting,John Nobile,Bernard P. Puc,David Light,Travis A. Clark,Martin Huber,Jeffrey T. Branciforte,Isaac B. Stoner,Simon Cawley,Michael R. Lyons,Yutao Fu,Nils Homer,Marina Sedova,Xin Miao,Brian Reed,Jeffrey Sabina,Erika Feierstein,Michelle Schorn,Mohammad Alanjary,Eileen T. Dimalanta,Devin Dressman,Rachel Kasinskas,Tanya Sokolsky,Jacqueline A. Fidanza,Eugeni Namsaraev,Kevin McKernan,Alan Williams,G. Thomas Roth,James Bustillo +43 more
TL;DR: A DNA sequencing technology in which scalable, low-cost semiconductor manufacturing techniques are used to make an integrated circuit able to directly perform non-optical DNA sequencing of genomes, showing its robustness and scalability by producing ion chips with up to 10 times as many sensors and sequencing a human genome.
Journal ArticleDOI
High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number
Benjamin J. Hindson,Kevin D. Ness,Donald A. Masquelier,Phillip Belgrader,Nicholas J. Heredia,Anthony J. Makarewicz,Isaac J. Bright,Michael Y. Lucero,Amy L. Hiddessen,Tina C. Legler,Tyler K. Kitano,Michael R. Hodel,Jonathan Petersen,Paul Wyatt,Erin R. Steenblock,Pallavi Shah,Luc J. Bousse,Camille B. Troup,Jeffrey Clark Mellen,Dean K. Wittmann,Nicholas G. Erndt,Thomas H. Cauley,Ryan T. Koehler,Austin P. So,Simant Dube,Klint Rose,Luz Montesclaros,Shenglong Wang,David P. Stumbo,Shawn Hodges,Steven Romine,Fred P. Milanovich,Helen E. White,John F. Regan,George Karlin-Neumann,Christopher Hindson,Serge Saxonov,Bill W. Colston +37 more
TL;DR: A high-throughput droplet digital PCR system that enables processing of ∼2 million PCR reactions using conventional TaqMan assays with a 96-well plate workflow is described that will allow researchers to explore complex genetic landscapes, discover and validate new disease associations, and define a new era of molecular diagnostics.
Journal ArticleDOI
Dynamic pattern formation in a vesicle-generating microfluidic device.
TL;DR: It is shown that a microfluidic device designed to produce reverse micelles can generate complex, ordered patterns as it is continuously operated far from thermodynamic equilibrium.
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