Enteric glia express proteolipid protein 1 and are a transcriptionally unique population of glia in the mammalian nervous system
Meenakshi Rao,Meenakshi Rao,Bradlee Nelms,Lauren Dong,Viviana Salinas-Rios,Michael Rutlin,Michael D. Gershon,Gabriel Corfas,Gabriel Corfas,Gabriel Corfas +9 more
TLDR
Enteric glia are transcriptionally unique and distinct from other cell types in the nervous system, suggesting that a combinatorial code of molecular markers can be used to identify distinct subtypes.Abstract:
In the enteric nervous system (ENS), glia outnumber neurons by 4-fold and form an extensive network throughout the gastrointestinal tract. Growing evidence for the essential role of enteric glia in bowel function makes it imperative to understand better their molecular marker expression and how they relate to glia in the rest of the nervous system. We analyzed expression of markers of astrocytes and oligodendrocytes in the ENS and found, unexpectedly, that proteolipid protein 1 (PLP1) is specifically expressed by glia in adult mouse intestine. PLP1 and S100β are the markers most widely expressed by enteric glia, while glial fibrillary acidic protein expression is more restricted. Marker expression in addition to cellular location and morphology distinguishes a specific subpopulation of intramuscular enteric glia, suggesting that a combinatorial code of molecular markers can be used to identify distinct subtypes. To assess the similarity between enteric and extraenteric glia, we performed RNA sequencing analysis on PLP1-expressing cells in the mouse intestine and compared their gene expression pattern to that of other types of glia. This analysis shows that enteric glia are transcriptionally unique and distinct from other cell types in the nervous system. Enteric glia express many genes characteristic of the myelinating glia, Schwann cells and oligodendrocytes, although there is no evidence of myelination in the murine ENS. GLIA 2015;63:2040-2057.read more
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Lineage-dependent gene expression programs influence the immune landscape of colorectal cancer.
Hae Ock Lee,Hae Ock Lee,Yourae Hong,Yourae Hong,Hakki Emre Etlioglu,Yong Beom Cho,Yong Beom Cho,Valentina Pomella,Ben Van den Bosch,Jasper Vanhecke,Sara Verbandt,Hyekyung Hong,Jae Woong Min,Nayoung K.D. Kim,Nayoung K.D. Kim,Hye Hyeon Eum,Hye Hyeon Eum,Junbin Qian,Bram Boeckx,Diether Lambrechts,Petros Tsantoulis,Gert De Hertogh,Woosung Chung,Taeseob Lee,Taeseob Lee,Minae An,Minae An,Hyun Tae Shin,Je-Gun Joung,Min Hyeok Jung,Gunhwan Ko,Pratyaksha Wirapati,Seok-Hyung Kim,Hee Cheol Kim,Seong Hyeon Yun,Iain Beehuat Tan,Iain Beehuat Tan,Bobby Ranjan,Woo Yong Lee,Tae-You Kim,Jung Kyoon Choi,Young Joon Kim,Shyam Prabhakar,Sabine Tejpar,Woong-Yang Park,Woong-Yang Park +45 more
TL;DR: The collective view of the cellular landscape and intercellular interactions in colorectal cancer provide mechanistic information for the design of efficient immuno-oncology treatment strategies.
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Adult enteric nervous system in health is maintained by a dynamic balance between neuronal apoptosis and neurogenesis
Subhash Kulkarni,Maria-Adelaide Micci,Jenna Leser,Changsik Shin,Shiue-Cheng Tang,Ya-Yuan Fu,Liansheng Liu,Qian Li,Monalee Saha,Cuiping Li,Grigori Enikolopov,Grigori Enikolopov,Laren Becker,Nikolai Rakhilin,Nikolai Rakhilin,Michael G. Anderson,Xiling Shen,Xiling Shen,Xinzhong Dong,Manish J. Butte,Hongjun Song,E. Michelle Southard-Smith,Raj P. Kapur,Milena Bogunovic,Pankaj J. Pasricha +24 more
TL;DR: Significance The demonstration of a robust neurogenesis program in the adult gut and the existence of an enteric neural precursor cell (ENPC) responsible for the same has profound biological and clinical implications.
Journal ArticleDOI
Neuronal programming by microbiota regulates intestinal physiology
Yuuki Obata,Álvaro Castaño,Stefan Boeing,Ana Carina Bon-Frauches,Candice Fung,Todd Fallesen,Mercedes Gomez de Agüero,Bahtiyar Yilmaz,Rita Lopes,Almaz Huseynova,Stuart Horswell,Muralidhara Rao Maradana,Werend Boesmans,Werend Boesmans,Pieter Vanden Berghe,Andrew J. Murray,Brigitta Stockinger,Andrew J. Macpherson,Vassilis Pachnis +18 more
TL;DR: This work demonstrates that the intrinsic neural networks of the colon exhibit unique transcriptional profiles that are controlled by the combined effects of host genetic programs and microbial colonization, and identifies AHR signalling in enteric neurons as a regulatory node that integrates the luminal environment with the physiological output of intestinal neural circuits to maintain gut homeostasis and health.
Journal ArticleDOI
Gut-Innervating Nociceptor Neurons Regulate Peyer’s Patch Microfold Cells and SFB Levels to Mediate Salmonella Host Defense
Nicole Y. Lai,Melissa A. Musser,Melissa A. Musser,Felipe A. Pinho-Ribeiro,Pankaj Baral,Amanda Jacobson,Pingchuan Ma,David E. Potts,Zuojia Chen,Zuojia Chen,Donggi Paik,Salima Soualhi,Yiqing Yan,Aditya Misra,Kaitlin Goldstein,Valentina N. Lagomarsino,Anja Nordstrom,Kisha N. Sivanathan,Antonia Wallrapp,Vijay K. Kuchroo,Roni Nowarski,Michael N. Starnbach,Hailian Shi,Hailian Shi,Neeraj K. Surana,Dingding An,Chuan Wu,Chuan Wu,Jun R. Huh,Meenakshi Rao,Isaac M. Chiu +30 more
TL;DR: It is found that nociceptor neurons critically mediate host defense against the bacterial pathogen Salmonella enterica serovar Typhimurium (STm), and a major role is revealed in sensing and defending against enteric pathogens.
Journal ArticleDOI
Lineage-dependent spatial and functional organization of the mammalian enteric nervous system
Reena Lasrado,Werend Boesmans,Werend Boesmans,Jens Kleinjung,Carmen Pin,Donald M. Bell,Leena Bhaw,Sarah McCallum,Hui Zong,Liqun Luo,Hans Clevers,Pieter Vanden Berghe,Vassilis Pachnis +12 more
TL;DR: It is shown that the amorphous neuroglia networks of the mouse ENS are composed of overlapping clonal units founded by postmigratory neural crest–derived progenitors, which underpin the organization of the peripheral nervous system.
References
More filters
Journal ArticleDOI
NIH Image to ImageJ: 25 years of image analysis
TL;DR: The origins, challenges and solutions of NIH Image and ImageJ software are discussed, and how their history can serve to advise and inform other software projects.
Journal ArticleDOI
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
Journal ArticleDOI
Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles
Aravind Subramanian,Pablo Tamayo,Vamsi K. Mootha,Sayan Mukherjee,Benjamin L. Ebert,Michael A. Gillette,Amanda G. Paulovich,Scott L. Pomeroy,Todd R. Golub,Eric S. Lander,Jill P. Mesirov +10 more
TL;DR: The Gene Set Enrichment Analysis (GSEA) method as discussed by the authors focuses on gene sets, that is, groups of genes that share common biological function, chromosomal location, or regulation.
Journal ArticleDOI
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks
Cole Trapnell,Adam Roberts,Loyal A. Goff,Loyal A. Goff,Loyal A. Goff,Geo Pertea,Daehwan Kim,Daehwan Kim,David R. Kelley,David R. Kelley,Harold Pimentel,Steven L. Salzberg,John L. Rinn,John L. Rinn,Lior Pachter +14 more
TL;DR: This protocol begins with raw sequencing reads and produces a transcriptome assembly, lists of differentially expressed and regulated genes and transcripts, and publication-quality visualizations of analysis results, which takes less than 1 d of computer time for typical experiments and ∼1 h of hands-on time.
Journal ArticleDOI
A robust and high-throughput Cre reporting and characterization system for the whole mouse brain
Linda Madisen,Theresa A. Zwingman,Susan M. Sunkin,Seung Wook Oh,Hatim A. Zariwala,Hong Gu,Lydia Ng,Richard D. Palmiter,Michael Hawrylycz,Allan R. Jones,Ed S. Lein,Hongkui Zeng +11 more
TL;DR: A set of Cre reporter mice with strong, ubiquitous expression of fluorescent proteins of different spectra is generated and enables direct visualization of fine dendritic structures and axonal projections of the labeled neurons, which is useful in mapping neuronal circuitry, imaging and tracking specific cell populations in vivo.