Glypican-1 identifies cancer exosomes and detects early pancreatic cancer
Sonia A. Melo,Linda B. Luecke,Christoph Kahlert,Agustín F. Fernández,Seth T. Gammon,Judith Kaye,Valerie S. LeBleu,Elizabeth A. Mittendorf,Juergen Weitz,Nuh N. Rahbari,Christoph Reissfelder,Christian Pilarsky,Mario F. Fraga,David Piwnica-Worms,Raghu Kalluri +14 more
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TLDR
GPC1+ crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy.Abstract:
Exosomes are lipid-bilayer-enclosed extracellular vesicles that contain proteins and nucleic acids. They are secreted by all cells and circulate in the blood. Specific detection and isolation of cancer-cell-derived exosomes in the circulation is currently lacking. Using mass spectrometry analyses, we identify a cell surface proteoglycan, glypican-1 (GPC1), specifically enriched on cancer-cell-derived exosomes. GPC1(+) circulating exosomes (crExos) were monitored and isolated using flow cytometry from the serum of patients and mice with cancer. GPC1(+) crExos were detected in the serum of patients with pancreatic cancer with absolute specificity and sensitivity, distinguishing healthy subjects and patients with a benign pancreatic disease from patients with early- and late-stage pancreatic cancer. Levels of GPC1(+) crExos correlate with tumour burden and the survival of pre- and post-surgical patients. GPC1(+) crExos from patients and from mice with spontaneous pancreatic tumours carry specific KRAS mutations, and reliably detect pancreatic intraepithelial lesions in mice despite negative signals by magnetic resonance imaging. GPC1(+) crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy.read more
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Recent achievements in exosomal biomarkers detection by nanomaterials-based optical biosensors - A review.
Baoyi Shao,Zhongdang Xiao +1 more
TL;DR: The review has focused on the progress and advancements in different optical-transducing approaches (Surface-Enhanced Raman Scattering, Surface Plasmon Resonance, Colorimetry, Immunochromatographic assay, Chemiluminescence, Electrochemilumitescence, and fluorescence) for detecting and profiling exosomal biomarkers.
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The molecular biology of pancreatic adenocarcinoma: translational challenges and clinical perspectives.
Shun Wang,Yan Zheng,Feng Yang,Le Zhu,Xiao-Qiang Zhu,Zhefang Wang,Xiaolin Wu,Chenghui Zhou,Jia-Yan Yan,Bei-Yuan Hu,Bo Kong,Deliang Fu,Christiane Bruns,Yue Zhao,Lun-Xiu Qin,Qiongzhu Dong +15 more
TL;DR: In this article, the authors summarized the biological features of pancreatic cancer and its metabolic reprogramming as well as the tumor microenvironment regulate its development and progression and discussed potential biomarkers for pancreatic diagnosis, prediction, and surveillance based on novel liquid biopsies.
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Advances in pancreatic cancer biomarkers.
TL;DR: Current and future biomarkers that has potential serve as critical tools for early diagnostic, predictive and prognostic indications in pancreatic cancer are reviewed.
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A protein corona-enabled blood test for early cancer detection
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TL;DR: The aim of this study is the development of a new pancreatic cancer diagnostic technology based on the exploitation of the nano-bio-interactions between nanoparticles and blood samples, which found that the protein corona of Pancreatic cancer patients was much more enriched than that of healthy individuals.
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