Glypican-1 identifies cancer exosomes and detects early pancreatic cancer
Sonia A. Melo,Linda B. Luecke,Christoph Kahlert,Agustín F. Fernández,Seth T. Gammon,Judith Kaye,Valerie S. LeBleu,Elizabeth A. Mittendorf,Juergen Weitz,Nuh N. Rahbari,Christoph Reissfelder,Christian Pilarsky,Mario F. Fraga,David Piwnica-Worms,Raghu Kalluri +14 more
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TLDR
GPC1+ crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy.Abstract:
Exosomes are lipid-bilayer-enclosed extracellular vesicles that contain proteins and nucleic acids. They are secreted by all cells and circulate in the blood. Specific detection and isolation of cancer-cell-derived exosomes in the circulation is currently lacking. Using mass spectrometry analyses, we identify a cell surface proteoglycan, glypican-1 (GPC1), specifically enriched on cancer-cell-derived exosomes. GPC1(+) circulating exosomes (crExos) were monitored and isolated using flow cytometry from the serum of patients and mice with cancer. GPC1(+) crExos were detected in the serum of patients with pancreatic cancer with absolute specificity and sensitivity, distinguishing healthy subjects and patients with a benign pancreatic disease from patients with early- and late-stage pancreatic cancer. Levels of GPC1(+) crExos correlate with tumour burden and the survival of pre- and post-surgical patients. GPC1(+) crExos from patients and from mice with spontaneous pancreatic tumours carry specific KRAS mutations, and reliably detect pancreatic intraepithelial lesions in mice despite negative signals by magnetic resonance imaging. GPC1(+) crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy.read more
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Feasibility of urinary extracellular vesicle proteome profiling using a robust and simple, clinically applicable isolation method.
Irene V. Bijnsdorp,Olga Maxouri,Aarzo Kardar,Tim Schelfhorst,Sander R. Piersma,Thang V. Pham,André N. Vis,R. Jeroen A. van Moorselaar,Connie R. Jimenez +8 more
TL;DR: The urinary EV isolation protocol using the Vn96-peptide is easier, time convenient and no special equipment is needed, in contrast to ultracentrifugation protocol, making this protocol clinically feasible and allows large-scale protein profiling of urinary EV biomarkers.
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Urinary Exosomal and cell-free DNA Detects Somatic Mutation and Copy Number Alteration in Urothelial Carcinoma of Bladder
Dong Hyeon Lee,Hana Yoon,Sanghui Park,Jeong Seon Kim,Young Ho Ahn,Kihwan Kwon,Donghwan Lee,Kwang Hyun Kim +7 more
TL;DR: Assessment of the availability of cell-free DNA and exosomal DNA in urine as a source for liquid biopsy in UBC and identified somatic mutations and CNV using urinary cfDNA and exoDNA found it to be another source forLiquid biopsy.
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The miR-92a-2-5p in exosomes from macrophages increases liver cancer cells invasion via altering the AR/PHLPP/p-AKT/β-catenin signaling.
Guodong Liu,Guodong Liu,Xiwu Ouyang,Xiwu Ouyang,Yin Sun,Yao Xiao,Yao Xiao,Bosen You,Yuan Gao,Shuyuan Yeh,Yixiong Li,Chawnshang Chang,Chawnshang Chang +12 more
TL;DR: Macrophages in the liver cancer tumor microenvironment may function via exosomes to regulate liver cancer progression, and targeting this newly identified macrophages/exosomes-miR-92a-2-5p/AR/PHLPP/p-AKT/β-catenin signaling may help in the development of novel treatment strategies to better suppress liver cancer progress.
Journal ArticleDOI
Glycosylation of Cancer Extracellular Vesicles: Capture Strategies, Functional Roles and Potential Clinical Applications
TL;DR: In this paper, the application of extracellular vesicles glycosylation in the development of novel EV detection and capture methodologies is discussed. And the authors highlight the potential of EV glyco-activation in the clinical setting for both cancer biomarker discovery and EV therapeutic delivery strategies.
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Plasma exosomes from endometrial cancer patients contain LGALS3BP to promote endometrial cancer progression
TL;DR: It is proposed that plasma exosomes containing LGALS3BP contributed to EC growth and angiogenesis during EC progression, which provided a novel perspective on EC diagnosis and prognosis.
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