Glypican-1 identifies cancer exosomes and detects early pancreatic cancer
Sonia A. Melo,Linda B. Luecke,Christoph Kahlert,Agustín F. Fernández,Seth T. Gammon,Judith Kaye,Valerie S. LeBleu,Elizabeth A. Mittendorf,Juergen Weitz,Nuh N. Rahbari,Christoph Reissfelder,Christian Pilarsky,Mario F. Fraga,David Piwnica-Worms,Raghu Kalluri +14 more
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TLDR
GPC1+ crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy.Abstract:
Exosomes are lipid-bilayer-enclosed extracellular vesicles that contain proteins and nucleic acids. They are secreted by all cells and circulate in the blood. Specific detection and isolation of cancer-cell-derived exosomes in the circulation is currently lacking. Using mass spectrometry analyses, we identify a cell surface proteoglycan, glypican-1 (GPC1), specifically enriched on cancer-cell-derived exosomes. GPC1(+) circulating exosomes (crExos) were monitored and isolated using flow cytometry from the serum of patients and mice with cancer. GPC1(+) crExos were detected in the serum of patients with pancreatic cancer with absolute specificity and sensitivity, distinguishing healthy subjects and patients with a benign pancreatic disease from patients with early- and late-stage pancreatic cancer. Levels of GPC1(+) crExos correlate with tumour burden and the survival of pre- and post-surgical patients. GPC1(+) crExos from patients and from mice with spontaneous pancreatic tumours carry specific KRAS mutations, and reliably detect pancreatic intraepithelial lesions in mice despite negative signals by magnetic resonance imaging. GPC1(+) crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy.read more
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Protein content and functional characteristics of serum-purified exosomes from patients with colorectal cancer revealed by quantitative proteomics.
TL;DR: It is demonstrated that SPEs of CRC patients play a pivotal role in promoting the tumor invasiveness, but have minimal influence on putative alterations in tumor survival or proliferation.
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Application of liquid biopsy in precision medicine: opportunities and challenges.
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Peng Zhang,Xin Zhou,Yong Zeng +2 more
TL;DR: An integrated, 3D nano-engineered exosome profiling platform (ExoProfile chip) was developed to afford ultrasensitive, multiplexed detection of a panel of surface protein markers on circulating exosomes with a minuscular volume of plasma.
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Serum exosomal microRNAs combined with alpha-fetoprotein as diagnostic markers of hepatocellular carcinoma.
Yurong Wang,Yurong Wang,Chunyan Zhang,Pengjun Zhang,Guanghong Guo,Jiang Tao,Xiumei Zhao,Jingjing Jiang,Xueliang Huang,Hongli Tong,Tian Yaping,Tian Yaping +11 more
TL;DR: The data suggest that serum exosomal microRNAs signature or their combination with traditional biomarker may be used as a suitable peripheral screening tool for HCC.
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New Approaches to SCLC Therapy: From the Laboratory to the Clinic
John T. Poirier,Julie George,Taofeek K. Owonikoko,Anton Berns,Elisabeth Brambilla,Lauren Averett Byers,David P. Carbone,Huanhuan Joyce Chen,Camilla L. Christensen,Caroline Dive,Anna F. Farago,Ramaswamy Govindan,Christine L. Hann,Matthew D. Hellmann,Leora Horn,Jane E. Johnson,Young Seok Ju,Sumin Kang,Mark A. Krasnow,James L. Lee,Se-Hoon Lee,Jonathan M. Lehman,Benjamin H. Lok,Christine M. Lovly,David MacPherson,David G. McFadden,John D. Minna,Matthew G. Oser,Keunchil Park,Kwon-Sik Park,Yves Pommier,Vito Quaranta,Neal Ready,Julien Sage,Giorgio V. Scagliotti,Martin L. Sos,Kate D. Sutherland,William D. Travis,Christopher R. Vakoc,Sarah J. Wait,Ignacio I. Wistuba,Kwok-Kin Wong,Hua Zhang,Jillian B. Daigneault,Jacinta Wiens,Charles M. Rudin,Trudy G. Oliver +46 more
TL;DR: This review largely summarizes work presented at the Third Biennial IASLC Small Cell Lung Cancer Meeting and identifies key knowledge gaps that should be addressed in order to advance the field in pursuit of reduced small cell lung cancer mortality.
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