Glypican-1 identifies cancer exosomes and detects early pancreatic cancer
Sonia A. Melo,Linda B. Luecke,Christoph Kahlert,Agustín F. Fernández,Seth T. Gammon,Judith Kaye,Valerie S. LeBleu,Elizabeth A. Mittendorf,Juergen Weitz,Nuh N. Rahbari,Christoph Reissfelder,Christian Pilarsky,Mario F. Fraga,David Piwnica-Worms,Raghu Kalluri +14 more
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TLDR
GPC1+ crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy.Abstract:
Exosomes are lipid-bilayer-enclosed extracellular vesicles that contain proteins and nucleic acids. They are secreted by all cells and circulate in the blood. Specific detection and isolation of cancer-cell-derived exosomes in the circulation is currently lacking. Using mass spectrometry analyses, we identify a cell surface proteoglycan, glypican-1 (GPC1), specifically enriched on cancer-cell-derived exosomes. GPC1(+) circulating exosomes (crExos) were monitored and isolated using flow cytometry from the serum of patients and mice with cancer. GPC1(+) crExos were detected in the serum of patients with pancreatic cancer with absolute specificity and sensitivity, distinguishing healthy subjects and patients with a benign pancreatic disease from patients with early- and late-stage pancreatic cancer. Levels of GPC1(+) crExos correlate with tumour burden and the survival of pre- and post-surgical patients. GPC1(+) crExos from patients and from mice with spontaneous pancreatic tumours carry specific KRAS mutations, and reliably detect pancreatic intraepithelial lesions in mice despite negative signals by magnetic resonance imaging. GPC1(+) crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy.read more
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Exosome-mediated siRNA delivery to suppress postoperative breast cancer metastasis
TL;DR: Results indicate that CBSA/siS100A4@Exosome self-assembled nanoparticles are a promising strategy to suppress postoperative breast cancer metastasis.
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A framework for the development of effective anti-metastatic agents.
Robin L. Anderson,Robin L. Anderson,Theo Balasas,Juliana Callaghan,R. Charles Coombes,Jeff Evans,Jacqueline A. Hall,Sally A. Kinrade,David Jones,Paul S. Jones,Robert Jones,John Marshall,Maria Beatrice Panico,Jacqui Shaw,Patricia S. Steeg,Mark T. Sullivan,Warwick Tong,Andrew D. Westwell,James W. A. Ritchie +18 more
TL;DR: The challenges associated with discovering and developing anticancer agents designed specifically to prevent or delay the metastatic outgrowth of cancer are described and guidance on how these challenges might be overcome is provided.
Journal ArticleDOI
Exosomes confer chemoresistance to pancreatic cancer cells by promoting ROS detoxification and miR-155-mediated suppression of key gemcitabine-metabolising enzyme, DCK.
Girijesh Kumar Patel,Mohammad Aslam Khan,Arun Bhardwaj,Sanjeev K. Srivastava,Haseeb Zubair,Mary C. Patton,Seema Singh,Moh’d Khushman,Ajay P. Singh +8 more
TL;DR: A novel role of exosomes in mediating the acquired chemoresistance of PC cells is established and either the functional suppression of miR-155 or restoration of DCK led to marked abrogation of Gem-Exo-mediatedChemoresistance.
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Immunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma
Priyanka Sharma,Sonja Ludwig,Sonja Ludwig,Laurent Muller,Laurent Muller,Chang Sook Hong,John M. Kirkwood,Soldano Ferrone,Theresa L. Whiteside +8 more
TL;DR: An immunoaffinity-based method for MTEX capture from plasma of melanoma patients is developed and separated MTEX from non-tumour cell-derived exosomes and evaluated the protein cargo of both fractions by quantitative flow cytometry.
Journal ArticleDOI
Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro-/nano-based devices
TL;DR: This Critical Review explores the emerging use of micro and nano-technology to isolate and detect exosomes and microvesicles in clinical samples and the application of this technology to the monitoring and diagnosis of cancer.
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