Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes
Lorenzo Galluzzi,Lorenzo Galluzzi,Lorenzo Galluzzi,Stuart A. Aaronson,John M. Abrams,Emad S. Alnemri,David W. Andrews,Eric H. Baehrecke,Nicolas G. Bazan,Mikhail V. Blagosklonny,Klas Blomgren,Klas Blomgren,Christoph Borner,Dale E. Bredesen,Dale E. Bredesen,Catherine Brenner,Maria Castedo,Maria Castedo,Maria Castedo,John A. Cidlowski,Aaron Ciechanover,Gerald M. Cohen,V De Laurenzi,R De Maria,Mohanish Deshmukh,Brian David Dynlacht,Wafik S. El-Deiry,Richard A. Flavell,Richard A. Flavell,Simone Fulda,Carmen Garrido,Carmen Garrido,Pierre Golstein,Pierre Golstein,Pierre Golstein,Marie-Lise Gougeon,Douglas R. Green,Hinrich Gronemeyer,Hinrich Gronemeyer,Hinrich Gronemeyer,György Hajnóczky,J. M. Hardwick,Michael O. Hengartner,Hidenori Ichijo,Marja Jäättelä,Oliver Kepp,Oliver Kepp,Oliver Kepp,Adi Kimchi,Daniel J. Klionsky,Richard A. Knight,Sally Kornbluth,Sharad Kumar,Beth Levine,Beth Levine,Stuart A. Lipton,Enrico Lugli,Frank Madeo,Walter Malorni,Jean-Christophe Marine,Seamus J. Martin,Jan Paul Medema,Patrick Mehlen,Patrick Mehlen,Gerry Melino,Gerry Melino,Ute M. Moll,Ute M. Moll,Eugenia Morselli,Eugenia Morselli,Eugenia Morselli,Shigekazu Nagata,Donald W. Nicholson,Pierluigi Nicotera,Gabriel Núñez,Moshe Oren,Josef M. Penninger,Shazib Pervaiz,Marcus E. Peter,Mauro Piacentini,Jochen H. M. Prehn,Hamsa Puthalakath,Gabriel A. Rabinovich,Rosario Rizzuto,Cecília M. P. Rodrigues,David C. Rubinsztein,Thomas Rudel,Luca Scorrano,Hans-Uwe Simon,Hermann Steller,Hermann Steller,J. Tschopp,Yoshihide Tsujimoto,Peter Vandenabeele,Ilio Vitale,Ilio Vitale,Ilio Vitale,Karen H. Vousden,Richard J. Youle,Junying Yuan,Boris Zhivotovsky,Guido Kroemer,Guido Kroemer,Guido Kroemer +103 more
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TLDR
A nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls is provided and the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cells is emphasized.Abstract:
Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios Thus far, dozens of methods have been proposed to quantify cell death-related parameters However, no guidelines exist regarding their use and interpretation, and nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate Here, we provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls These guidelines are intended for investigators who study cell death, as well as for reviewers who need to constructively critique scientific reports that deal with cellular demise Given the difficulties in determining the exact number of cells that have passed the point-of-no-return of the signaling cascades leading to cell death, we emphasize the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cellsread more
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Stress responses in flavivirus-infected cells: activation of unfolded protein response and autophagy.
Ana-Belén Blázquez,Estela Escribano-Romero,Teresa Merino-Ramos,Juan-Carlos Saiz,Miguel A. Martín-Acebes +4 more
TL;DR: This review addresses the current knowledge of the relationship between endoplasmic reticulum stress, UPR, and autophagy in flavivirus-infected cells and the growing evidences for an involvement of these cellular pathways in the replication and pathogenesis of these viruses.
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Prognostic and Predictive Value of DAMPs and DAMP-Associated Processes in Cancer
Jitka Fucikova,Irena Moserova,Linda Urbanova,Lucillia Bezu,Oliver Kepp,Isabelle Cremer,Cyril Salek,P. Strnad,Guido Kroemer,Lorenzo Galluzzi,Radek Spisek +10 more
TL;DR: Clinical data indicating that DAMPs and DAMP-associated stress responses might have prognostic or predictive value for cancer patients are reviewed.
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Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity
Yiwen Lu,Qiyi Zhao,Jian-You Liao,Erwei Song,Qidong Xia,Jiayao Pan,Yihong Li,Jiaqian Li,Boxuan Zhou,Yingying Ye,Can Di,Shubin Yu,Yunjie Zeng,Shicheng Su +13 more
TL;DR: It is shown that ICOSL in B cells boosts anti-tumor immunity by enhancing the effector to regulatory T cell ratio and that CD55, a complement inhibitory protein, determines the opposite roles of B cells in chemotherapy.
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TBK1 kinase addiction in lung cancer cells is mediated via autophagy of Tax1bp1/Ndp52 and non-canonical NF-κB signalling.
Alice C. Newman,Caroline L. Scholefield,Alain J. Kemp,Michelle Newman,Edward G. McIver,Ahmad Kamal,Simon Wilkinson +6 more
TL;DR: It is demonstrated that the xenophagy-associated kinase TBK1 drives basal autophagy, consistent with its known requirement in K-Ras-dependent NSCLC proliferation, and proposed that this TBK 1-dependent mechanism for NF-κB signalling contributes to autophagic addiction in K.Ras driven NSCLCs.
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Elevated α-synuclein caused by SNCA gene triplication impairs neuronal differentiation and maturation in Parkinson's patient-derived induced pluripotent stem cells.
Luís M. A. Oliveira,Lisandro J. Falomir-Lockhart,Michelle G. Botelho,K-H Lin,Pauline Wales,Jan C. Koch,Ellen Gerhardt,Holger Taschenberger,Holger Taschenberger,Tiago F. Outeiro,Paul Lingor,Birgitt Schüle,Donna J. Arndt-Jovin,Thomas M. Jovin +13 more
TL;DR: It is concluded that a two-fold overexpression of α-synuclein caused by a triplication of the SNCA gene is sufficient to impair the differentiation of neuronal progenitor cells, a finding with implications for adult neurogenesis and Parkinson’s disease progression, particularly in the context of bioenergetic dysfunction.
References
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Santos A. Susin,Hans K. Lorenzo,Naoufal Zamzami,Isabel Marzo,Bryan E. Snow,Joan Mangion,Etienne Jacotot,Paola Costantini,Markus Loeffler,Nathanael Larochette,David R. Goodlett,Ruedi Aebersold,David P. Siderovski,Josef M. Penninger,Guido Kroemer +14 more
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Mitochondrial Membrane Permeabilization in Cell Death
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