Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes
Lorenzo Galluzzi,Lorenzo Galluzzi,Lorenzo Galluzzi,Stuart A. Aaronson,John M. Abrams,Emad S. Alnemri,David W. Andrews,Eric H. Baehrecke,Nicolas G. Bazan,Mikhail V. Blagosklonny,Klas Blomgren,Klas Blomgren,Christoph Borner,Dale E. Bredesen,Dale E. Bredesen,Catherine Brenner,Maria Castedo,Maria Castedo,Maria Castedo,John A. Cidlowski,Aaron Ciechanover,Gerald M. Cohen,V De Laurenzi,R De Maria,Mohanish Deshmukh,Brian David Dynlacht,Wafik S. El-Deiry,Richard A. Flavell,Richard A. Flavell,Simone Fulda,Carmen Garrido,Carmen Garrido,Pierre Golstein,Pierre Golstein,Pierre Golstein,Marie-Lise Gougeon,Douglas R. Green,Hinrich Gronemeyer,Hinrich Gronemeyer,Hinrich Gronemeyer,György Hajnóczky,J. M. Hardwick,Michael O. Hengartner,Hidenori Ichijo,Marja Jäättelä,Oliver Kepp,Oliver Kepp,Oliver Kepp,Adi Kimchi,Daniel J. Klionsky,Richard A. Knight,Sally Kornbluth,Sharad Kumar,Beth Levine,Beth Levine,Stuart A. Lipton,Enrico Lugli,Frank Madeo,Walter Malorni,Jean-Christophe Marine,Seamus J. Martin,Jan Paul Medema,Patrick Mehlen,Patrick Mehlen,Gerry Melino,Gerry Melino,Ute M. Moll,Ute M. Moll,Eugenia Morselli,Eugenia Morselli,Eugenia Morselli,Shigekazu Nagata,Donald W. Nicholson,Pierluigi Nicotera,Gabriel Núñez,Moshe Oren,Josef M. Penninger,Shazib Pervaiz,Marcus E. Peter,Mauro Piacentini,Jochen H. M. Prehn,Hamsa Puthalakath,Gabriel A. Rabinovich,Rosario Rizzuto,Cecília M. P. Rodrigues,David C. Rubinsztein,Thomas Rudel,Luca Scorrano,Hans-Uwe Simon,Hermann Steller,Hermann Steller,J. Tschopp,Yoshihide Tsujimoto,Peter Vandenabeele,Ilio Vitale,Ilio Vitale,Ilio Vitale,Karen H. Vousden,Richard J. Youle,Junying Yuan,Boris Zhivotovsky,Guido Kroemer,Guido Kroemer,Guido Kroemer +103 more
Reads0
Chats0
TLDR
A nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls is provided and the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cells is emphasized.Abstract:
Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios Thus far, dozens of methods have been proposed to quantify cell death-related parameters However, no guidelines exist regarding their use and interpretation, and nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate Here, we provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls These guidelines are intended for investigators who study cell death, as well as for reviewers who need to constructively critique scientific reports that deal with cellular demise Given the difficulties in determining the exact number of cells that have passed the point-of-no-return of the signaling cascades leading to cell death, we emphasize the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cellsread more
Citations
More filters
Journal ArticleDOI
Mycobacterium tuberculosis -induced miR-155 subverts autophagy by targeting ATG3 in human dendritic cells
Marilena P. Etna,Alessandro Sinigaglia,Angela Grassi,Elena Giacomini,Alessandra Romagnoli,Manuela Pardini,Martina Severa,Melania Cruciani,Fabiana Rizzo,Eleni Anastasiadou,Barbara Di Camillo,Luisa Barzon,Gian Maria Fimia,Riccardo Manganelli,Eliana M. Coccia +14 more
TL;DR: How Mtb can manipulate cellular miRNA expression to regulate Atg3 for its own survival is suggested, and the importance to develop novel therapeutic strategies against tuberculosis that would boost autophagy is highlighted.
Journal ArticleDOI
The flavonoid 4,4′-dimethoxychalcone promotes autophagy-dependent longevity across species
Didac Carmona-Gutierrez,Andreas Zimmermann,Andreas Zimmermann,Katharina Kainz,Federico Pietrocola,Guo Chen,Silvia Maglioni,Alfonso Schiavi,Jihoon Nah,Sara Mertel,Christine B. Beuschel,Francesca Castoldi,Valentina Sica,Gert Trausinger,Reingard Raml,Cornelia Sommer,Sabrina Schroeder,Sebastian J. Hofer,Maria A. Bauer,Tobias Pendl,Jelena Tadic,Christopher Dammbrueck,Zehan Hu,Christoph Ruckenstuhl,Tobias Eisenberg,Sylvère Durand,Sylvère Durand,Noélie Bossut,Noélie Bossut,Fanny Aprahamian,Fanny Aprahamian,Mahmoud Abdellatif,Simon Sedej,David Enot,David Enot,Heimo Wolinski,Jörn Dengjel,Oliver Kepp,Christoph Magnes,Frank Sinner,Frank Sinner,Thomas R. Pieber,Thomas R. Pieber,Junichi Sadoshima,Natascia Ventura,Stephan J. Sigrist,Stephan J. Sigrist,Guido Kroemer,Frank Madeo +48 more
TL;DR: It is demonstrated that DMC extends lifespan and promotes health in multiple organisms by inducing autophagy and is identified in the plant Angelica keiskei koidzumi, to which longevity- and health-promoting effects are ascribed in Asian traditional medicine.
Journal ArticleDOI
KCNQ1OT1 promotes autophagy by regulating miR-200a/FOXO3/ATG7 pathway in cerebral ischemic stroke.
TL;DR: It is revealed that potassium voltage‐gated channel subfamily Q member 1 opposite strand 1 (KCNQ1OT1) was significantly upregulated in ischemic stroke and offers a potential novel strategy for isChemic stroke therapy.
Journal ArticleDOI
The fine tuning of metabolism, autophagy and differentiation during in vitro myogenesis
Paola Fortini,C Ferretti,Egidio Iorio,Monica Cagnin,L Garribba,Donatella Pietraforte,Mario Falchi,Barbara Pascucci,Sara Baccarini,Federica Morani,Suratchanee Phadngam,G. De Luca,Ciro Isidoro,Eugenia Dogliotti +13 more
TL;DR: It is demonstrated that basal autophagy contributes to a correct execution of myogenesis and that physiological p53 activity is required for muscle homeostasis by regulating metabolism and by affectingAutophagy and differentiation.
Journal ArticleDOI
Bergamot polyphenol fraction prevents nonalcoholic fatty liver disease via stimulation of lipophagy in cafeteria diet-induced rat model of metabolic syndrome
Maddalena Parafati,Maddalena Parafati,Antonella Lascala,Valeria Maria Morittu,Francesca Trimboli,Antonia Rizzuto,Elvira Brunelli,Francesca Coscarelli,Nicola Costa,Domenico Britti,James Ehrlich,Ciro Isidoro,Vincenzo Mollace,Vincenzo Mollace,Elzbieta Janda,Elzbieta Janda +15 more
TL;DR: It is demonstrated that the liver and its lipid metabolism are the main targets of bergamot flavonoids, supporting the concept that supplementation of BPF is an effective strategy to prevent NAFLD.
References
More filters
Journal ArticleDOI
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.
TL;DR: The extent of tissue-PCD revealed by this method is considerably greater than apoptosis detected by nuclear morphology, and thus opens the way for a variety of studies.
Journal ArticleDOI
The Release of Cytochrome c from Mitochondria: A Primary Site for Bcl-2 Regulation of Apoptosis
TL;DR: In a cell-free apoptosis system, mitochondria spontaneously released cytochrome c, which activated DEVD-specific caspases, leading to fodrin cleavage and apoptotic nuclear morphology, and Bcl-2 acts to inhibit cy tochrome c translocation, thereby blocking caspase activation and the apoptotic process.
Journal ArticleDOI
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
TL;DR: It is shown here that this morphological change is closely associated with excision of nucleosome chains from nuclear chromatin, apparently through activation of an intracellular, but non-lysosomal, endonuclease.
Journal ArticleDOI
Molecular characterization of mitochondrial apoptosis-inducing factor
Santos A. Susin,Hans K. Lorenzo,Naoufal Zamzami,Isabel Marzo,Bryan E. Snow,Joan Mangion,Etienne Jacotot,Paola Costantini,Markus Loeffler,Nathanael Larochette,David R. Goodlett,Ruedi Aebersold,David P. Siderovski,Josef M. Penninger,Guido Kroemer +14 more
TL;DR: The identification and cloning of an apoptosis-inducing factor, AIF, which is sufficient to induce apoptosis of isolated nuclei is reported, indicating that AIF is a mitochondrial effector of apoptotic cell death.
Journal ArticleDOI
Mitochondrial Membrane Permeabilization in Cell Death
TL;DR: Once MMP has been induced, it causes the release of catabolic hydrolases and activators of such enzymes (including those of caspases) from mitochondria, meaning that mitochondria coordinate the late stage of cellular demise.
Related Papers (5)
Classification of cell death: recommendations of the Nomenclature Committee on Cell Death
Guido Kroemer,Guido Kroemer,Guido Kroemer,Lorenzo Galluzzi,Lorenzo Galluzzi,Lorenzo Galluzzi,Peter Vandenabeele,John M. Abrams,Emad S. Alnemri,Eric H. Baehrecke,Mikhail V. Blagosklonny,Wafik S. El-Deiry,Pierre Golstein,Pierre Golstein,Douglas R. Green,Michael O. Hengartner,Richard A. Knight,Sharad Kumar,Stuart A. Lipton,Stuart A. Lipton,Stuart A. Lipton,Walter Malorni,Gabriel Núñez,Marcus E. Peter,Juerg Tschopp,Junying Yuan,Mauro Piacentini,Boris Zhivotovsky,Gerry Melino,Gerry Melino +29 more