Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
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Journal ArticleDOI
Screening of Plant Extracts for Antioxidant Activity: a Comparative Study on Three Testing Methods
TL;DR: Three methods widely employed in the evaluation of antioxidant activity, namely 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, static headspace gas chromatography (HS-GC) and beta-carotene bleaching test (BCBT), have been compared with regard to their application in the screening of plant extracts.
Journal ArticleDOI
Genesis and development of DPPH method of antioxidant assay
Sagar B. Kedare,Rashmi Singh +1 more
TL;DR: A critical review on various developments to the DPPH method is presented, which has undergone various modifications to suit the requirements, even though the basic approach remains same in all of them.
Journal ArticleDOI
Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies
TL;DR: In this article, a b-carotene-linoleic acid (linoleate) model system was used to evaluate the scavenging effect on the DPPH free radical and capacity to scavenge hydroxyl free radicals.
Journal ArticleDOI
Antioxidant activities of Sechium edule (Jacq.) Swartz extracts
TL;DR: The antioxidant properties of Sechium edule extracts were tested by three established in vitro methods, namely reducing power, β-carotene linoleate model and 1,1- diphenyl-2 picrylhydrazyl (DPPH) radical-scavenging.
Journal ArticleDOI
Comparison of the free radical-scavenging activity of propolis from several regions of Argentina
TL;DR: It was observed that all samples of ethanolic extracts of propolis showed free radical-scavenging activity in terms of scavenging of the radical DPPH but the highest activities were found for samples from Tucumán and Santiago del Estero, which may justify their use as a source of natural antioxidants.
References
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TL;DR: A relatively simple but sensitive and reliable method for quantifying the oxygen radical absorbance capacity (ORAC) of antioxidants in biological tissues has been automated for use with the COBAS FARA II centrifugal analyzer with a fluorescence-measuring attachment.
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Enhanced chemiluminescent assay for antioxidant capacity in biological fluids
TL;DR: Whitehead and Thorpe as discussed by the authors used an enhanced chemiluminescent reaction involving horseradish peroxidase and luminol for the detection of antioxidants and measurements of antioxidants capacity.
Journal ArticleDOI
Inhibition of Human Low-Density Lipoprotein Oxidation in Relation to Composition of Phenolic Antioxidants in Grapes (Vitis vinifera)
TL;DR: In this paper, the authors investigated the antioxidant properties of phenolic extracts from fourteen different types of fresh grapes by measuring the inhibition of human low-density lipoprotein (LDL) oxidation in vitro.
Journal ArticleDOI
Varietal Differences in the Phenolic Content and Superoxide Radical Scavenging Potential of Wines from Different Sources
Michikatsu Sato,Narasimhan Ramarathnam,Yumiko Suzuki,Tetsuro Ohkubo,Masao Takeuchi,Hirotomo Ochi +5 more
TL;DR: In this paper, the superoxide radical scavenging potential of wines was analyzed for total phenolic content, total and free sulfur dioxide contents, and superoxide radicals scavenging ability.