Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
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Antioxidant Activities of Extract and Fractions from the Hypocotyls of the Mangrove Plant Kandelia candel
TL;DR: Among the extract and fractions, the LF fraction exhibits the best antioxidant performance and contains a large number of procyanidins and a small amount of prodelphinidins, and the epicatechin is the main extension unit.
Journal ArticleDOI
Manganese oxide nanoparticle-induced changes in growth, redox reactions and elicitation of antioxidant metabolites in deadly nightshade (Atropa belladonna L.)
TL;DR: In this article, the potential effects of Mn2O3 NPs on morphology, physiology and secondary metabolism of shoot tip cultures in the medicinal plant deadly nightshade (Atropa belladonna).
Journal ArticleDOI
Evaluation of antioxidant capacity and flavor profile change of pomegranate wine during fermentation and aging process
TL;DR: Fermentation significantly reduced the relative content of aldehydes, ketones, heterocyclic and aromatic compounds, but promoted the generation of esters and alcohols, which may provide a promising instruction for improving functional features and quality control of the pomegranate wine.
Journal ArticleDOI
Evaluation of antioxidant activities of 3 edible mushrooms: Ramaria flava (Schaef.: Fr.) Quél., Rhizopogon roseolus (Corda) T.M. Fries., and Russula delica Fr.
TL;DR: The methanolic extracts of Ramaria flava, Rhizopogon roseolus, and Russula delica were analyzed for their antioxidant activities in different test systems including β-carotene/linoleic acid, 11-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, reducing power, and metal chelating activities in addition to their total phenolic and flavonoid contents.
Journal ArticleDOI
Antioxidant Capacity and Cytotoxicity of Essential Oil and Methanol Extract of Aniba canelilla (H.B.K.) Mez
Joyce Kelly R. da Silva,Pergentino José da Cunha Sousa,Eloisa Helena A. Andrade,José Guilherme S. Maia +3 more
TL;DR: The leaves and fine stems, bark, and trunk wood oils of Aniba canelilla showed yields ranging from 0.2 to 1.3%, and in the brine shrimp bioassay the values of lethal concentration and DPPH radical scavenging activity were lower than that of the wood methanol extracts, showing significant biological activities.
References
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Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.