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Journal ArticleDOI

HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl

TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.
Abstract
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.

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Evaluation of possible mechanisms of Cordia dichotoma fruits for hyperlipidemia controlling in Wistar albino rats

TL;DR: The protective effect of C. dichotoma extract against hyperlipidemia may be attributed to the reduced ability of an animal to ingest and absorb fat and cholesterol, and enhanced ability to get rid of them in faecal excretions.
Journal ArticleDOI

Phytochemical Analysis and Antifungal Bioactivity of Pulicaria undulata (L.) Methanolic Extract and Essential Oil

TL;DR: In this paper, the aerial parts of P. undulata plants were collected from the Red sea coastal desert during the flowering stage and used for phytochemical analysis, which revealed the occurrence of ten bio-active phytocomponents including cyclotrisiloxane, hexamethyl-, cyclotetrasiloxideane, octamethyl, cyclopentasiloxANE, decamethyldecane, dodecanoic acid, methyl ester, tetrafluorophthalonitrile, gamma-Sitosterol, nonacos

ACTIVIDAD ANTIoXIDANTE DE TRES ECoTIPoS DE MACA (LEpIDIUM MEYENII wALp) TRATADA CoN RADIACIÓN GAMMA

TL;DR: In this paper, various doses (0-20 kGy) of gamma radiation have been applied to samples of maca (Lepidium meyenii walp) flour, yellow, white and purple ecotypes, to decontaminate them.
References
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Journal ArticleDOI

Antioxidant Determinations by the Use of a Stable Free Radical

TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.

TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI

Wine, alcohol, platelets, and the French paradox for coronary heart disease

TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI

The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen

TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI

Antioxidant activity of various tea extracts in relation to their antimutagenicity

TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.
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