Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
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Optimization of Curcuminoids Extraction for Evaluation Against Parkinson’s Disease in Rats
Manal A. Hamed,Mona A. Mohammed,Asmaa F. Aboul Naser,Azaa A. Matloub,Dalia B. Fayed,Sanaa A. Ali,Wagdy K. B. Khalil +6 more
TL;DR: As the plant extract improved DA and adenosine A2A gene expression levels, this shed the light on its role as a therapeutic dopaminergic agent and therefore its mode of action must be studied in details.
Journal ArticleDOI
Identification of a characteristic antioxidant, anthrasesamone F, in black sesame seeds and its accumulation at different seed developmental stages.
TL;DR: The radical scavenging assay showed that anthrasesamone F had more potent activity than Trolox and was localized in the seed coat of black seeds and accumulated after the seed Coat color changed to black.
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An antioxidant potential of hydromethanolic extract of urtica parviflora roxb.
TL;DR: The study indicated that Urtica parviflora could protect the cell injury caused by the reactive oxygen species and might be a valuable source of antioxidant both for medicine and food industry.
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Identification, quantification and antioxidant activity of secondary metabolites in leaf and callus extracts of coleus forskohlii
TL;DR: In this paper, sixteen combinations of NAA+BAP, and NAA + KN, used in the study, induced callus from leaves of Coleus forskohlii with a variable response, 0.5mg / l NAA plus 1.5 mg / l BAP was the most efficient (98.8 ± 1.1).
Journal ArticleDOI
Antioxidant activity and phenolic compounds in conventional and organic red grapes (var. Monastrell)
TL;DR: In this paper, a comparison of the phenolic composition and antioxidant activity between red grapes (Vitis vinifera) var “Monastrell” obtained from organic and conventional cultures has been studied.
References
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Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
Journal ArticleDOI
Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.