Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
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Antioxidant and free radical scavenging activities of pigments extracted from molasses alcohol wastewater
TL;DR: The results showed that the macroporous resin HPD-600 was most appropriate for extracting the pigments from molasses alcohol wastewater as discussed by the authors, and the scavenging effect of pigment extract on superoxide anion radical was very weak with IC50 value greater than 10mg/ml.
Journal ArticleDOI
Antioxidant activity of lactic-fermented Chinese cabbage
TL;DR: In this article, fermented cabbage prepared by a dry-salt method was first extracted with water and methanol, and the effect of fermentation on the change of antioxidant activity, total phenolic and total flavonoid content was also investigated.
Journal ArticleDOI
Changes in antioxidant capacity during development and ripening of goldenberry (Physalis peruviana L.) fruit and in response to 1-methylcyclopropene treatment
TL;DR: In this article, the effect of 1-methylcyclopropene (1-MCP, 0.2μL −L −1 ), a blocker of the ethylene receptor, and ethylene (2000μL L −1 Ethrel) were tested to elucidate the role of ethylene on the antioxidant capacity of this fruit during ripening and storage.
Journal ArticleDOI
Flow injection based methods for fast screening of antioxidant capacity.
TL;DR: Different approaches to flow injection of antioxidants based on scavenging of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical cation (ABTS(+)) or 2, 2-diphenyl-1-picrylhydrazyl radical (DDPH) or based on the determination of total reducing capacity are compared to critically compare.
Journal ArticleDOI
Evaluation of the Antioxidant Activity of Three Varieties of Honey from Different Botanical and Geographical Origins
Hasan A. Alzahrani,Laid Boukraa,Yuva Bellik,Fatiha Abdellah,Balkees A. Bakhotmah,Sevgi Kolayli,Huseyin Sahin +6 more
TL;DR: A strong correlation between the antioxidant activities of honeys and their total phenol contents has been noticed, and it is speculated that these compounds may strengthen the organism defenses and consequently prevent oxidative stress in humans.
References
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Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
Journal ArticleDOI
Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.