Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
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Monthly and Yearly Variations in Oxygen Radical-scavenging Activity, Ascorbic Acid Content, and Degrees Brix of Komatsuna (Brassica rapa var. perviridis) in the Kanto Region, Japan
Journal ArticleDOI
In-vitro radical scavenging activity of Daucus carota L. extracts
Nermeen M Arafa,Usama I Aly +1 more
TL;DR: In this article, the authors evaluated the antioxidant potential of methanol extracts of Daucus carota L. callus addend with an amino acid precursor, l-phenylalanine, under light and dark conditions.
Journal ArticleDOI
Effect of Enzyme-Treated Radish Leaves on Lipid Metabolism in Rats Fed a High-Fat Diet
TL;DR: Results indicate that supplementation with enzyme-treated radish leaves increase the useful fiber contents and may have a pronounced impact on lipid metabolism of serum and liver in rats fed a high fat diet.
Journal Article
Evaluación de la actividad antioxidante de extractos de Palicourea guianensis (Rubiaceae)
TL;DR: In this article, the authors evaluated the antioxidant activity of plant Palicourea guianensis (Rubiaceae), for which extraction was done by the aerial part of the species with methanol as the primary solvent, after this, the crude extract was fractionated with the following solvents: n-hexane, dichloromethane, ethyl acetate and water.
Journal ArticleDOI
Appraisal of maize germplasm for identification of nutritionally rich nutraceutical genotypes
Ramanjeet Kaur,K. Kaur +1 more
TL;DR: Thirty maize genotypes were characterized for their nutritional, antinutritional and nutraceutical traits for identification of elite germplasm rich in beneficial characteristics and it may be concluded that CML134, CML266, TOO14601, LM11,CML264, C ML321, SE563, LM10, LM18, LM14 and CML32 were nutritionally rich nutr pharmaceutical genotypes having low antinUTrient potency.
References
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Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
Journal ArticleDOI
Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.