Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
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Effects of conventional and ultrasound treatments on physicochemical properties and antioxidant capacity of floral honeys from Northern Thailand
TL;DR: In this article, three different honey samples viz. longan flower, lychee flower and wildflower honeys were collected from Northern Thailand and subjected to conventional thermal (90°C/5min) and ultrasound (40% and 80% amplitudes/20 kHz/30min) treatments.
Journal ArticleDOI
Bio-based films prepared with soybean by-products and pine (Pinus densiflora) bark extract
Yingying Han,Miao Yu,Lijuan Wang +2 more
TL;DR: In this paper, bio-based antioxidant films were prepared by incorporating different concentrations of pine (Pinus densiflora) bark extract (PBE) into soy protein isolate (SPI) matrix and analyzed in terms of structure, as well as physical, mechanical, and antioxidant properties.
Journal ArticleDOI
Cationic phosphorus dendrimers and therapy for Alzheimer's disease
Tomasz Wasiak,Monika Marcinkowska,Ireneusz Pieszyński,Maria Zablocka,Anne-Marie Caminade,Jean-Pierre Majoral,Barbara Klajnert-Maculewicz,Barbara Klajnert-Maculewicz +7 more
TL;DR: The results presented suggest that phosphorus dendrimers may be considered in the future as agents in AD therapy, and should demonstrate anti-inflammatory properties, inhibit acetylcholine hydrolysis, and have antioxidant capacity.
Journal ArticleDOI
Effect of Seasonal Variations and Collection Form on Antioxidant Activity of Propolis from San Juan, Argentina
María Inés Isla,Iris Catiana Zampini,Roxana Mabel Ordóñez,Soledad Cuello,Belén Carrasco Juárez,Jorge Esteban Sayago,María Inés Nieva Moreno,María Rosa Alberto,Nancy Vera,E. Bedascarrasbure,Alejandro Raul Alvarez,Fabián Cioccini,Luis Maldonado +12 more
TL;DR: The results suggest that Argentine propolis from Cuyo is a promising source of bioactive compounds as ingredients for developing functional foods with a beneficial impact on oxidative stress.
Journal ArticleDOI
Free radical scavenging activity in the nonenzymatic fraction of human saliva: a simple DPPH assay showing the effect of physical exercise.
TL;DR: In this article, the authors used 1,1′-diphenyl-2-picrylhydrazyl (DPPH) to estimate free radical scavenging activity in the low-molecular-weight nonenzymatic fraction (LMNEF) of human whole saliva.
References
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Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.