Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
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Jaboticaba (Plinia trunciflora (O. Berg) Kausel) fruit reduces oxidative stress in human fibroblasts cells (MRC-5)
Caroline Calloni,Rafaela Dall Agnol,Luana Soares Martínez,Fábio de Siqueira Marcon,Sidnei Moura,Mirian Salvador +5 more
TL;DR: Findings show a new role for jaboticaba as a mitochondrial protectant in pathological conditions where mitochondrial dysfunction is involved and its in vitro antioxidant activity was evaluated.
Journal ArticleDOI
Investigation of albumin properties in patients with chronic renal failure.
TL;DR: Results show that haemodialysis provoked significant changes in conformational properties of plasma albumin, which resulted in the loss of its biological functions, and suggest that oxidative stress and glycation of proteins in plasma are developed during haemmodialysis.
Journal ArticleDOI
Study on biological activities of Physalis alkekengi var. francheti polysaccharide.
TL;DR: In this paper, the results of animal experiments showed that D-galactose-induced aging in mice, as evidenced by alterations in superoxide dismutase, glutathione peroxidase, catalase and monoamine oxidase activities and malondialdehyde level, was ameliorated by FP treatment.
Journal ArticleDOI
Essential oil composition and antioxidant capacity of Lippia schomburgkiana.
da Silva Na,da Silva Jk,Eloisa Helena A. Andrade,Léa Maria Medeiros Carreira,Pergentino José da Cunha Sousa,J. G. S. Maia +5 more
TL;DR: The sensory evaluation of the leaves of L. schomburgkiana showed a percentage acceptance value very close to commercial oregano, indicating that the plant can be used in spice and condiment products.
Journal ArticleDOI
Antioxidant Activity of the Essential Oil and Oleoresin of Zingiber Officinale Roscoe as Affected by Chemical Environment
Yuva Bellik,Fadila Benabdesselam,A. Ayad,Zina Dahmani,Laid Boukraa,Abderrahim Nemmar,Mokrane Iguer-Ouada +6 more
TL;DR: In this article, three different biochemical test systems were chosen based on their solubility to study the antioxidant activity of ginger extracts: reducing power, free radical scavenging and H2O2 scavenging assays.
References
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Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
Journal ArticleDOI
Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.