Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
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Determination of Antioxidant Activity and Characterization of Antioxidant Phenolics in the Plum Vinegar Extract of Cherry Blossom (Prunus lannesiana)
Ritaro Matsuura,Hironori Moriyama,Naruki Takeda,Kyoko Yamamoto,Yusuke Morita,Tomoko Shimamura,Hiroyuki Ukeda +6 more
TL;DR: Results showed that the plum vinegar extract of cherry blossom had a greater superoxide anion scavenging activity compared with red wine, which is a well-known strong antioxidant.
Journal ArticleDOI
Comparative analysis of solid-state bioprocessing and enzymatic treatment of finger millet for mobilization of bound phenolics
TL;DR: The present work investigates the probable bioprocessing technique to mobilize the bound phenolics naturally found in finger millet cell wall for enriching it with dietary antioxidants and establishes the most effective strategy.
Journal ArticleDOI
Antioxidant Activity of the Derivatives of Polysaccharide Extracted from a Chinese Medical Herb (Ramulus mori)
Zuofa Zhang,Jie Jin,Liangen Shi +2 more
TL;DR: In this paper, the antioxidant activity of the polysaccharide from Ramulus mori and its derivatives (sulfated, phosphorylated, acetylated and benzoylated) was determined, including scavenging activity against superoxide, hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals, reducing power and chelating ability.
Journal ArticleDOI
The antioxidative activity of traditional Japanese herbs.
TL;DR: The extracts of gennoshoko, yomogi and iwa‐tobacco showed remarkable radical scavenging activity, and the volatile oil of ymogi obtained by distillation or steeping in organic solvents had especially strong antioxidative activity.
Journal ArticleDOI
Constituents of leaves of Phellodendron japonicum MAXIM. and their antioxidant activity.
Chih-Yang Chiu,Chia Ying Li,Chao-Chen Chiu,Masatake Niwa,Susumu Kitanaka,Amooru G. Damu,E-Jian Lee,Tian Shung Wu +7 more
TL;DR: Three new flavonoid derivatives and thirty known compounds have been isolated from the leaves of Phellodendron japonicum MAXIM by means of spectroscopic analysis, including extensive 2D NMR and Mass spectra.
References
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Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
Journal ArticleDOI
Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.