scispace - formally typeset
Journal ArticleDOI

HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl

TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.
Abstract
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.

read more

Citations
More filters
Journal ArticleDOI

Potential Bioconversion of Microalgal Waste for Carotenoid-Rich Biomass Production

TL;DR: It is worth to note that microalgal wastes substrate can be used as an alternative carbon and nitrogen sources for the cultivation of R. mucilaginous AY-01 and contribute to the enhancement of the economic competition of biodiesel production from microalgae.
Journal ArticleDOI

Enhanced antifungal activity of Piper betle against candidiasis infection causing Candida Albicans and In silico analysis with its virulent protein

TL;DR: The present study reveals that methanol and ethanol extract of P. betle is a potential source of natural-free radical scavenging antioxidants and will be great helpful in the new drug analysis for the determination of antimicrobial biocompounds against candidiasis and other clinically related infections.
Journal ArticleDOI

Edible Solanum trilobatum chloroform extract modulates CCL 4 induced toxic changes in rat liver by enhancing the antioxidant activity

TL;DR: H hepatoprotective and antioxidant activity of the polyphenol rich chloroform extract of Solanum trilobatum was evaluated on CCl4 induced acute hepatotoxicity in Sprague Dawley rats and clearly demonstrates that CST contains therapeutic components which in combination/alone possess hepatoprotsective and in vivo antioxidant effect.
Journal ArticleDOI

SIFAT PRO-OKSIDAN SARI JERUK NIPIS (Citrus aurentifolia) TERHADAP AKTIFITAS ANTIOKSIDAN TEH HIJAU (Camellia sinensis)

TL;DR: In this paper, the effect of addition of extract citrus on the antioxidative activity of green tea and to determine the optimum temperature at which to extract green tea to get the best antioxidative activities was investigated.
Journal ArticleDOI

Synthesis, DFT calculations, In silico studies, and biological evaluation of pyrano[2,3-c]pyrazole and pyrazolo[4′,3′:5,6]pyrano[2,3-d]pyrimidine derivatives

TL;DR: In this article , a series of new pyrano[2,3- c ]pyrazole and pyrazolo[4′,3′:5,6]pyrano-pyrimidine derivatives were synthesized using facile green approach in excellent yields.
References
More filters
Journal ArticleDOI

Antioxidant Determinations by the Use of a Stable Free Radical

TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
Journal ArticleDOI

Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.

TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI

Wine, alcohol, platelets, and the French paradox for coronary heart disease

TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI

The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen

TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI

Antioxidant activity of various tea extracts in relation to their antimutagenicity

TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.
Related Papers (5)