Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
More filters
Journal ArticleDOI
Diversity in antioxidant properties and mineral contents of Allium paradoxum in the Hyrcanian forests, Northern Iran
TL;DR: There is a remarkable and significant variation of antioxidant activities among different studied populations of A. paradoxum in the Hyrcanian forests.
Journal ArticleDOI
Investigation of phytochemical and antimicrobial properties of Linum usitatissimum in presence of ZnO/Zn(OH)2 nanoparticles and extraction of euphol from Euphorbia microsciadia
Mehrorang Ghaedi,Masoomeh Yousefi-Nejad,Mohammad Safarpoor,Shima Hashemi,Alireza Goudarzi,Inderjeet Tyagi,Shilpi Agarwal,Vinod Kumar Gupta +7 more
TL;DR: The effect of plant extract with ZnO/Zn(OH)2 nanoparticles suspension on bacteria and antioxidants content of the extracts were also deter...
Journal ArticleDOI
Biological activities and nutritional value of Tapinanthus bangwensis leaves
TL;DR: The cytotoxic and antioxidant properties of the ethanolic extract and fractions of the leaves of T. bangwensis were evaluated in this article, where the authors investigated their total phenol and flavonoid contents, proximate analysis and their mineral composition.
Journal ArticleDOI
Grafting of oxidized carboxymethyl cellulose with hydrogen peroxide in presence of Cu(II) to chitosan and biological elucidation
TL;DR: In this article, the chemical interaction of chitosan (CS) is performed in the presence of sodium carboxymethyl cellulose (CMC) and/or oxidized CMC.
Journal ArticleDOI
2,2 -Diphenyl-1-picrylhydrazil free radical scavenging activity of antioxidant mixtures evaluated by response surface methodology
Inar Alves de Castro,Marcelo Macedo Rogero,Roberto M. Junqueira,Mariana Magalhães Carrapeiro +3 more
TL;DR: This article applied the statistical technique known as response surface methodology (RSM) to model the free radical scavenging activity of mixtures of three antioxidant compounds, using 2,2-diphenyl-1-picrylhydrazyl (DPPH).
References
More filters
Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
Journal ArticleDOI
Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.