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Journal ArticleDOI

HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl

TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.
Abstract
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.

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Citations
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Effect of Phenolic Acid on Antioxidant Activity of Wine and Inhibition of Pectin Methyl Esterase

TL;DR: In this paper, the authors investigated the inhibitory mechanism of gallic acid and coumaric acid on pectin methyl esterase (PME) and found that gallic acids and Coumaric acids were mixed inhibitors of PME.
Journal ArticleDOI

Recovery of protein from discharged wastewater during the production of chitin

TL;DR: The obtained protein sediment contained abundant amino acids, especially isoleucine, methione and lysine that are absent in other protein resource.
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Growth and phytochemicals of ice plant (Mesembryanthemum crystallinum L.) as affected by various combined ratios of red and blue LEDs in a closed-type plant production system

TL;DR: In this article, a combination of red and blue light-emitting diodes (LEDs) was used to improve the growth and phytochemical contents of the ice plant in a closed-type plant production system.
Journal ArticleDOI

Synthesis of CuS nanoparticles and evaluation of its antimicrobial properties in combination with Linum usitatissimum root and shoot extract

TL;DR: The superposition of metal nanoparticles with the L. usitatissimum extracts was found to be effective in the eradication of the bacterial infections, and proved to be a good alternative of antibiotics.
Journal ArticleDOI

In vitro evaluation of antioxidant and antidiabetic activities of Syzygium densiflorum fruits

TL;DR: In this article, the authors provided experimental support for the traditional knowledge of Syzygium densiflorum (S. densiflamum ) fruits using a Soxhlet extractor.
References
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Journal ArticleDOI

Antioxidant Determinations by the Use of a Stable Free Radical

TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.

TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
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Wine, alcohol, platelets, and the French paradox for coronary heart disease

TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI

The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen

TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
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Antioxidant activity of various tea extracts in relation to their antimutagenicity

TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.
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