Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
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Identification of creatinine, the antioxidant in the non-absorbed fraction of dried bonito stock (Katsuodashi).
TL;DR: In this article, the authors show that 1H-NMR-13C NMR can be used for 13C-nMR, which is the state-of-the-art NMR technology.
Journal ArticleDOI
Secondary Metabolism-Inducing Treatments During in vitro Development of Turmeric (Curcuma longa L.) Rhizomes
TL;DR: Turmeric (Curcuma longa L.) plants were grown in vitro for 17 or 22 weeks as a fed-batch culture in 2.5 L vessels yielded 39 to 43 g and 62 to 70 g of fresh rhizomes per vessel, respectively (95 % confidence interval) as discussed by the authors.
Actividad antifúngica de antioxidantes derivados de cuatro cultivares de Capsicum spp. contra hongos fitopatógenos
Nancy Raquel López-Muñoz,Mirella Romero-Bastidas,Pablo Misael Arce-Amezquita,José Saúl Hernández-Rubio +3 more
TL;DR: El extracto de chile habanero es eficaz para controlar Cochliobollus spp.
Total phenolics, flavonoids and antioxidant activity of fresh water macroalgae from Ubon Ratchathani, Thailand
TL;DR: In this paper, three types of macro algaes were extracted from Ubon Ratchathani, Thailand with 95% ethanol and the results showed that the ethanolic extracts showed high DPPH free radical scavenging activity and antioxidant capacity.
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Dose-dependent Medicinal Effects of Thymus haussknechtii Velen Grown Wild in Turkey.
Hasan Kiliçgün,Mustafa Korkmaz +1 more
TL;DR: Thymus haussknechtii can be reliable antioxidant, antibacterial antifungal substance at concentrations of 4% when it is used as a supplement to therapeutic regimens and for medicinal purposes.
References
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Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.