Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
More filters
In-vitro antioxidant activity of the roots of melothria heterophylla (lour.) cogn.
TL;DR: In this article, the preliminary phytochemical analysis and antioxidant potential in different in-vitro models of different extracts (ethyl acetate, ethanol and aqueous) of Melothria heterophylla Lour Cogn root were investigated.
Journal Article
Synthesis and biological evaluation of novel 1,5-benzothiazepin-4(5H)-ones as potent antiangiogenic and antioxidant agents
Channegowda V. Deepu,Goravanahalli M. Raghavendra,Nanjappagowda D. Rekha,Kempegowda Mantelingu,Kanchugarakoppal S. Rangappa,D. G. Bhadregowda +5 more
TL;DR: Novel 1,5-benzothiazepin-4-(5H)-one derivatives (8a-8g) showed excellent free-radical scavenging activities in the metal ion-chelating assay, and compounds 8f and 8g showed nitric oxide radical scavenging properties at a concentration of 20-100 μg/mL, and are thus more potent than BHT.
Journal ArticleDOI
Bioactivities, heavy metal contents and toxicity effect of macroalgae from two sites in Madura, Indonesia
Sin War Naw,Sin War Naw,Nwet Darli Kyaw Zaw,Nwet Darli Kyaw Zaw,Nanik Siti Aminah,Mochammad Amin Alamsjah,Alfinda Novi Kristanti,Aondohemba Samuel Nege,Aondohemba Samuel Nege,Aondohemba Samuel Nege,Hnin Thanda Aung +10 more
TL;DR: Sargassum duplicatum and Padina tetrastromatica seaweed species collected from oil and a non-oil extraction site at Madura Island were investigated in this article for selected bioactivities, heavy metals and toxicity effects.
Journal ArticleDOI
Chemical Composition and Antioxidant Activity of Essential Oil and Hydrosol Extract Obtained by Hydrodistillation (HY) and Liquid–Liquid Extraction (LLE) of Psoralea bituminosa
Radia Lemouchi,Chaouki Selles,Mohammed El Amine Dib,Nassima Benmansour,Asma Allal,Boufeldja Tabti,Kheireddine Ouali +6 more
TL;DR: In this paper, the chemical composition of essential oil and hydrosol extract obtained by hydrodistillation and liquid-liquid extraction of Psoralea bituminosa was investigated using gas chromatography/retraction.
References
More filters
Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
Journal ArticleDOI
Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.