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Journal ArticleDOI

HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl

TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.
Abstract
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.

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Comparison of antioxidant and pro-oxidant activity in coffee beverages prepared with conventional and “Torrefacto” coffee

TL;DR: In this paper, the authors compared the antioxidant and pro-oxidant properties of coffee beverages obtained with conventional and torrefacto roasted coffee, and found that the latter has higher antioxidant capacity than the former.
Journal ArticleDOI

Thin Films of Liquid Media for Heterotrophic Growth and Storage Organ Development: Turmeric (Curcuma longa) as a Model Plant

TL;DR: The gentle rocking action of plantlets in sugar-containing liquid medium was demonstrated to produce functional storage organs and increased and improved biomass in liquid culture compared to agar was based on greater sugar use.
Journal Article

Identification of β-carotene and β-sitosterol in methanolic extract of Dipteracanthus patulus (Jacq) nees and their role in antimicrobial and antioxidant activity.

TL;DR: Experimental findings indicate promising antimicrobial activity (antibacterial and antifungal) and potent antioxidant activity of MEDP.
Journal ArticleDOI

Alterations in Red Blood Cells and Plasma Properties after Acute Single Bout of Exercise

TL;DR: It is shown that a single bout of exercise can lead to mobilization of defensive antioxidant systems in blood against oxidative stress in young untrained men.
Journal ArticleDOI

Gum acacia coating with garlic and cinnamon as an alternate, natural preservative for meat and fish

TL;DR: It is proven that spices like garlic and cinnamon that are used as regular ingredients in cooking can act as preservative due to their rich antibacterial and antioxidant profile.
References
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Journal ArticleDOI

Antioxidant Determinations by the Use of a Stable Free Radical

TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.

TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI

Wine, alcohol, platelets, and the French paradox for coronary heart disease

TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI

The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen

TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
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Antioxidant activity of various tea extracts in relation to their antimutagenicity

TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.
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