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Journal ArticleDOI

HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl

TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.
Abstract
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.

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Citations
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Journal ArticleDOI

Antimutagenic and antioxidant properties of milk-kefir and soymilk-kefir.

TL;DR: It is suggested that milk-kefir and soymilk-kefIR may be considered among the more promising food components in terms of preventing mutagenic and oxidative damage.
Journal ArticleDOI

Antioxidant activities of flavidin in different in vitro model systems.

TL;DR: This is the first report on antioxidant activity of 9,10-dihydro-5H-phenanthro-(4,5 bcd)-pyrans/flavidin type of compounds and the data obtained in the in vitro models clearly establish the antioxidant potency of flavidin.
Journal ArticleDOI

Antioxidant properties of papain hydrolysates of wheat gluten in different oxidation systems

TL;DR: In this article, membrane ultra-filtration was used to separate the peptides of wheat gluten into permeate (P) and retentate (5-K) fractions, and the antioxidative activity of the P fraction was almost the same as that of vitamin E at pH 7.0.

Antioxidant activities of flavidin in different in vitro model systems

TL;DR: In this paper, the antioxidant performance of 9,10-dihydro-5H-phenanthro-(4,5 bcd)-pyrans/flavidin type of compounds was investigated.
Journal ArticleDOI

Effect of Processing Techniques at Industrial Scale on Orange Juice Antioxidant and Beneficial Health Compounds

TL;DR: In this article, five processes at industrial scale (squeezing, mild pasteurization, standard pasteurisation, concentration, and freezing) used in commercial orange juice manufacturing were studied.
References
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Journal ArticleDOI

Antioxidant Determinations by the Use of a Stable Free Radical

TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.

TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI

Wine, alcohol, platelets, and the French paradox for coronary heart disease

TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI

The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen

TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI

Antioxidant activity of various tea extracts in relation to their antimutagenicity

TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.
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