Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
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Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate
TL;DR: The obtained results indicate that IS induces oxidative damage to hemolysate and plasma components, and suggests that the increased concentration of IS in patients with chronic kidney disease may lead to a decrease in the lifespan of erythrocytes in their bloodstream.
Journal ArticleDOI
Effect of green tea beverages on efficacy of povidone-iodine
Yoshitaka Tayama,Yoshitaka Tayama,Katsushi Miyake,Kazumi Sugihara,Shigeyuki Kitamura,Masao Kobayashi,Shushi Morita,Shigeru Ohta,Kenji Kihira +8 more
TL;DR: It is indicated that green tea beverages reduce the antibacterial activity of PVP-I in proportion to their antioxidant activity, and beverages with antioxidant activity should not be consumed immediately after P VP-I gargling.
Avaliação dos efeitos antitumorais da própolis vermelha em células humanas in vitro
TL;DR: A propolis vermelha brasileira tem atraido interesses cientificos e economicos devido as suas variadas atividades biologicas, sendo necessaria uma completa caracterizacao quimica para cada tipo de propolis, a fim of se elucidar os compostos presentes e responsaveis pela atividade biologica observada.
Journal ArticleDOI
Studies on inhibition of H2O2 induced TGF–β1 expression in peripheral blood mononuclear cells by novel pyridine appended lutein derivative
TL;DR: Pyridine appended Lutein showed higher DPPH radical scavenging activity and inhibited H2O2 induced TGF–β1 expression in peripheral blood mononuclear cells at low concentrations than unmodified LuteIn.
Antioxidant and free radical scavenging activities of Phellinus merrillii extracts
TL;DR: In this article, the authors investigated the possible antioxidant activity of different extracts of Phellinus merrillii (PM) and concluded that PM might contribute to its antiradical activities.
References
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Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
Journal ArticleDOI
Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.