Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
More filters
Micoproteínas de macrofungos obtidas em cultivo em estado sólido: avaliação do enriquecimento nutricional e compostos bioativos
TL;DR: Agaricus blazei, Auricularia fuscosuccinea, Pleurotus albidus, and Agaricus firenissi have been investigated in this paper.
Journal ArticleDOI
Effect of operational parameters of microwave vacuum drying combined with hot-air pre-drying to physical, sensory, and nutritional properties of dried apple snack
TL;DR: In this paper, the physical properties of products produced by microwave vacuum drying combined with hot-air pre-drying (CMVD) mainly depend on the specific energy input, which is associated with higher dry mass content.
Journal ArticleDOI
The Antioxidative Characteristics of Taro and Sweet Potato Protein Hydrolysates and Their Inhibitory Capability on Angiotensin Converting Enzyme
Chen-Yu Wu,Kuo-Wei Lin +1 more
TL;DR: In this article, the antioxidative capacities of taro and sweet potato proteins were analyzed and further fractionated into different molecular weight fractions, namely >10 kDa, 5'10kDa, 1'5kDa and <1kDa.
Journal Article
Evaluation of total phenolic contents and antioxidant activities in different solvent extracts of Diospyros melanoxylon Roxb. bark
TL;DR: In this paper, the anti-oxidative potential of phenolics present in different solvents were evaluated by DPPH free radical scavenging assay, O-phenanthroline assay and reducing power assay.
Journal ArticleDOI
RADICAL SCAVENGING ACTIVITY OF DECALPOLINE, A NOVEL COMPOUND CHARACTERIZED FROM Decalepis hamiltonii
TL;DR: In this article, the ethanol extract of Decalepis hamiltonii roots is subjected to antioxidant activity-guided fractionation by repeated silica gel column chromatography to get a pure antioxidant compound which was subjected to extensive analysis by UV, IR, LC-MS, and 2D HMQC NMR.
References
More filters
Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
Journal ArticleDOI
Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.