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Journal ArticleDOI

HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl

TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.
Abstract
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.

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Citations
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Encapsulation of extract prepared from irradiated onion scales in alginate beads: a potential functional food ingredient

TL;DR: In this paper, onion scales were irradiated and the effects of irradiation on their bioactive properties were studied and it was observed that alginate beads containing 6% OSE showed the maximum antioxidant activity.
Journal ArticleDOI

Protective effect of areca inflorescence extract on hydrogen peroxide-induced oxidative damage to human serum albumin

TL;DR: In this article, the authors investigated the effect of three areca inflorescence extracts (boiling water extract, ambient temperature water extract and ethanol extract) on hydrogen peroxide-induced oxidative damage to human serum albumin.
Journal ArticleDOI

Essential oil composition of Tanacetum kotschyi from Turkey

TL;DR: This work aims to provide a history of Pharmacognosy in Turkey and its applications in the context of modern medicine and its role in the response to infectious disease.
Journal ArticleDOI

Flow-injection analytical system for the evaluation of antioxidative activity

Hiroyuki Ukeda
- 01 Jan 2004 - 
TL;DR: In this article, the authors describe a 3-diphenyl-2-picrylhydrazyl-6-sulfonic acid (2,2'-azino-bis) and 3-benzthiazoline-6 sulfonyl sulfonic acid.
Journal ArticleDOI

Ultra-performance LC-photodiode array-eλ-ESI-MS/MS screening method for the detection of radical-scavenging natural antioxidants from radix et rhizoma Rhei

TL;DR: The use of the analytical screening method based on ultra-performance LC-photodiode array-el-ESI-MS/MS would provide a new way for rapid detection of radical-scavenging natural compounds from radix et rhizoma Rhei or complex matrices.
References
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Journal ArticleDOI

Antioxidant Determinations by the Use of a Stable Free Radical

TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.

TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI

Wine, alcohol, platelets, and the French paradox for coronary heart disease

TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI

The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen

TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
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Antioxidant activity of various tea extracts in relation to their antimutagenicity

TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.
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