Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
Citations
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Protective effects of bioactive phytochemicals from Mentha piperita with multiple health potentials
Seyedeh Maryam Sharafi,Iraj Rasooli,Parviz Owlia,Massoud Taghizadeh,Shakiba Darvish Alipoor Astaneh +4 more
TL;DR: The volatile oil displayed high cytotoxic action toward the human tumor cell line and may be exploited as a natural source of bioactive phytopchemicals bearing antimicrobial and antioxidant potentials that could be supplemented for both nutritional purposes and preservation of foods.
Journal ArticleDOI
Assessment of subcritical propane, ultrasound-assisted and Soxhlet extraction of oil from sweet passion fruit (Passiflora alata Curtis) seeds
Marlene G. Pereira,Fabiane Hamerski,Eriel Forville de Andrade,Agnes de Paula Scheer,Marcos L. Corazza +4 more
TL;DR: In this paper, the results of subcritical propane (SubFE), ultrassound-assisted and Soxhlet extraction of sweet passion fruit ( Passiflora alata Curtis) seed oil were reported in terms of extraction yield, refractive index, density, acid value, fatty acid composition, tocopherol content, antioxidant activity and oxidative stability.
Journal ArticleDOI
Free radical-scavenging capacity and inhibitory activity on rat erythrocyte hemolysis of feruloyl oligosaccharides from wheat bran insoluble dietary fiber
TL;DR: Data indicated that the water-soluble feruloyl oligosaccharides from wheat bran insoluble dietary fiber might have potential as natural antioxidants.
Journal ArticleDOI
Exogenous putrescine reduces flooding-induced oxidative damage by increasing the antioxidant properties of Welsh onion
TL;DR: In this article, the authors examined the role of putrescine (Put) in the response of Welsh onion plants to flooding stress and found that put pre-treatment increased the anti-oxidative activity of onion cells.
Journal ArticleDOI
Ultrasound-microwave assisted extraction of pectin from fig (Ficus carica L.) skin: Optimization, characterization and bioactivity
TL;DR: Four operating parameters in ultrasound-microwave assisted extraction (UMAE) process including sonication time, microwave power, irradiation time, and the liquid to solid ratio on the extraction yield of pectin from fig skin were optimized and a disintegrated network in structural morphology and the main functional groups was exhibited.
References
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Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.