Journal ArticleDOI
HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl
TLDR
An HPLC method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine, and the results well agree with those of previous reports.Abstract:
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rose wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.read more
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Dissertation
Studies on Bioprospecting of George Watt Listed Plants with Special Reference to West Bengal
Journal ArticleDOI
Enhancing the antioxidant activity of immature calamondin by heat treatment
TL;DR: In this article, the authors investigated the effect of heat treatment on antioxidant activity and phenolic compounds of immature calamondin (Citrus mitis Blanco) and concluded that the browning products resulted in an increase of the antioxidant activity of calamondins, while the increase of antioxidant activity was due to increased phenolic content.
Journal ArticleDOI
Antioxidative and antimicrobial activities of tigernut tubers phenolic extract
TL;DR: Phenolic compounds of tigernut tubers were extracted by methanol, colorimetrically determined, separated and identified using HPLC, and the results showed that total phenolic compounds recorded about 197.20 mg/100g and the methanolic yield was 1.02%.
Journal ArticleDOI
Evaluation of the antioxidant, anti-inflammatory, and antitumor properties of Sabal grown in Egypt
TL;DR: In this paper, the anti-inflammatory activity was investigated in-vitro using cyclooxygenase (COX)-1 and COX-2 inhibition assays.
Journal ArticleDOI
Study on Antioxidant Capacity and Phenolic Content of Various Parts of Wax Gourd (Benincasa hispida)
Noriham Abdullah,Syida Wan Kamarudin Wan Saidatul,Zainal Samicho,Khairusy Syakirah Zulkifli,Nurain Aziman +4 more
TL;DR: In this paper, the antioxidant capacity and total phenolic content of various part of wax gourd (Benincasa hispida) extracts were measured by three different assays such as scavenging activity, ferric reducing activity and -arotene bleaching assays.
References
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Journal ArticleDOI
Antioxidant Determinations by the Use of a Stable Free Radical
TL;DR: In this paper, the chemical nature of the antioxidant is known and a test specific for the compound or group of interest; for example, the nitroprusside test for sulphydryl groups.
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Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study.
TL;DR: Flavonoids in regularly consumed foods may reduce the risk of death from coronary heart disease in elderly men and showed an inverse relation with incidence of myocardial infarction.
Journal ArticleDOI
Wine, alcohol, platelets, and the French paradox for coronary heart disease
Serge Renaud,M de Lorgeril +1 more
TL;DR: Data from Caerphilly, Wales, show that platelet aggregation, which is related to CHD, is inhibited significantly by alcohol at levels of intake associated with reduced risk of CHD.
Journal ArticleDOI
The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen
TL;DR: The reduction of nitro blue tetrazolium (NitroBT) with NADH mediated by phenazine methosulfate (PMS) under aerobic conditions was inhibited upon addition ofsuperoxide dismutase, indicating the involvement of superoxide aninon radical in the reduction of NitroBT.
Journal ArticleDOI
Antioxidant activity of various tea extracts in relation to their antimutagenicity
Gow-Chin Yen,Hui-Yin Chen +1 more
TL;DR: The relationship between antioxidant activity and antimutagenicity of various tea extracts (green tea, pouchong tea, oolong tea and black tea) was investigated in this article, which showed that all tea extracts exhibited markedly antioxidant activity.